Principle

The FSTest Heavy Metal Cd Rapid Test Strip is based on immunochromatographic assay. The test strip has an area for result reading, which has a T (Test) line and C (Control) line. Both lines are invisible before testing. When the sample is applied to the loading side of the strip, the liquid will laterally flow upward through the surface of the test strip and react with the pre-coated recombinant antigens. If there is amount of heavy metal cadmium (Cd) in the sample, the intensity of T line will be inhibited and become relatively lighter than C line. Meanwhile the C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can indicate the presence of heavy metal cadmium (Cd) in the sample.

Limit of detection

Milk:50 μg/kg

Drinking water:25 μg/L

Kit composition

Test strips ×10

Microwells ×10

Heavy metal Cd chelating agent ×1

1.5 mL Centrifuge tubes x10

User manual.

Procedure & Steps

1. Pipette 150 μL of sample into a 1.5 mL centrifuge tube, add  450 μL of heavy metal Cd chelating agent, vortex to mix, and ready for the test.

2. Open the package tube, take out the test strips and microwells and make necessary labels or markings.

Note: Re-cap the tube immediately after usage to avoid contamination and moisture.

3. Pipette 150 μL of sample into the microwells pre-coated with reagent, mix slowly but thoroughly with pipette, then incubate for  3 min at room temperature (20-40℃).

4. Insert the test strips into the microwells and start timing.

5. Interpret the results in 10-13 min. Results are invalid after13 min.