1.  Principle

The FSTest Ochratoxin Rapid Test Kit is based onimmunochromato-graphic assay.  The test cassette has a testing window for the observation of assay running and result reading.  The testing window has an invisible T (test) line and a C (control) line before running the assay.  When the treated sample was applied intothe sample hole on the device, the liquid will laterally flow throughthe surface of the test strip and react with the pre-coatedrecombinant antigens.  If there are amount ochratoxin in the sample, The intensity of T line will be inhibited.  Meanwhile, the Cline should always appear after a sample is applied, which indicates a valid result.  By comparison the intensity between two lines, the device can indicate the presence of ochratoxin in the sample.  

2.  Application

Intended to test ochratoxin in grain. 

3.  Limit of detection

Grain (such as corn, wheat, rice, red beans, mung beans, etc.): 5μg/kg

4.  Kit composition

Test cassette ×10

Extracting solution (50 mL) ×1

Diluent solution (10 mL) ×1

2 mL centrifuge tubes ×10

10 mL centrifuge tubes ×10

User manual.

5.  Quantity

10tests/kit

6. Sample preparation

6.1. Take an appropriate amount of grain and grind it, weigh 1 g of sample and add it into a 10 mL centrifuge tube. 

6.2. Add 4 mL of extracting solution, re-cap the tube tightly, vortexfor 3 mins to mix, then centrifuge at 4000 rpm for 3 mins. 

6.3. Pipette 100 µL of supernatant into a 2 mL centrifuge tube, add100 µL of diluent solution, shake to mix

7. Procedure & Steps 

7.1. Before use, ensure the cassette is between 20 to 40℃. 

7.2. Take the test cassette out of the bag and put it on a flat surface. 

7.3. Pipette 100 μL of sample solution into the sample hole of the test cassette. 

7.4 Interpret the results after 8-10 mins. Results are invalid after 10 mins

8. Interpretation results