Difenoconazole Rapid Test Kit
Brand: FSTest
5–8 mins Rapid Readout Time | 0.02–15 mg/kg Tunable Matrix LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Difenoconazole Rapid Test Kit is a professional-grade rapid screening tool engineered for the qualitative detection of difenoconazole residues in fresh fruits and vegetables.
Difenoconazole is a widely monitored broad-spectrum triazole fungicide critical to global agricultural pest management and modern crop protection. By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural chemical extraction into a simplified room-temperature workflow, serving as an indispensable first-line defense in fresh produce logistics, harvesting hubs, and corporate quality compliance screening programs in just 5 to 8 minutes.
High-Speed Field Turnaround: Complete the entire screening assay workflow and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.
Dynamic Matrix-Specific Detection Limits: Calibrated to adapt flexibly across diverse food matrices, dynamically targeting regulatory cut-off thresholds ranging from an ultra-sensitive 0.02 mg/kg up to 15 mg/kg by adjusting sample supernatant dilution ratios.
Massive Fruit & Vegetable Adaptability: Officially validated across over 50 specific agricultural crops including potatoes, leafy greens, brassica, cucurbits, berries, citrus, stone fruits, pome fruits, and tropical commodities.
High Specificity & Zero Cross-Reactivity: Proven entirely unaffected by non-target agrochemicals up to 10 mg/L (including Chlorpyrifos, Chlorothalonil, Carbendazim, and Procymidone).
Safe & Non-Toxic Formulation: Supplied with a specialized extraction solution certified as safe, non-toxic, and user-friendly for operators processing samples on-site.
| Product Name | Difenoconazole Rapid Test Kit (Colloidal Gold) |
|---|---|
| Target Analyte Compound | Difenoconazole (CAS: 119446-68-3 | C₁₉H₁₇Cl₂N₃O₃ | 406.263 g/mol) |
| Detection Principle | Competitive Lateral Flow Immunochromatography |
| Pack Size | 10 tests / kit |
| Assay Run Time & Volume | 5–8 minutes (results invalid after 8 minutes); 100 µL final prepared solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Applicable Agricultural Commodity Matrix | Targeted LOD (mg/kg) | Supernatant X (µL) | Extracting Solution Y (µL) |
|---|---|---|---|
| Potato | 0.02 mg/kg | 100 µL | 0 µL |
| Asparagus, Wild Rice Stem | 0.03 mg/kg | 100 µL | 0 µL |
| Lily Bulb | 0.05 mg/kg | 100 µL | 0 µL |
| Sweet Potato, Pomegranate, Watermelon | 0.1 mg/kg | 50 µL | 200 µL |
| Pea | 0.15 mg/kg | 50 µL | 200 µL |
| Garlic, Cabbage, Cauliflower, Pickling Cucumber, Carrot, Mandarin, Tangerine, Orange, Plum, Mango, Papaya, Pineapple | 0.2 mg/kg | 50 µL | 200 µL |
| Zucchini, Ginger | 0.3 mg/kg | 50 µL | 200 µL |
| Broccoli, Onion, Tomato, Loofah, Green Bean, Apple, Pear, Hawthorn, Peach, Nectarine, Grape, Melon, Lychee | 0.5 mg/kg | 50 µL | 450 µL |
| Eggplant, Bell Pepper, Okra, Cherry Tomato, Lemon, Pomelo, Kumquat, Avocado | 0.6 mg/kg | 50 µL | 650 µL |
| Snow Pea, Hami Melon, Bailan Melon, Muskmelon, Pepino | 0.7 mg/kg | 50 µL | 850 µL |
| Chinese Cabbage, Pepper, Cucumber, Bitter Melon, Pumpkin, Banana | 1.0 mg/kg | 30 µL | 870 µL |
| Green Onion, Leaf Lettuce, Iceberg Lettuce, Dragon Fruit | 2.0 mg/kg | 10 µL | 990 µL |
| Celery, Strawberry | 3.0 mg/kg | 10 µL | 990 µL |
| Kiwifruit, Loquat, Jujube, Blueberry | 5.0 mg/kg | 10 µL | 1490 µL |
| Spinach, Indian Lettuce | 10.0 mg/kg | 10 µL | 1990 µL |
| Watercress (Special Two-Step Protocol below) | 15.0 mg/kg | 100 µL (Step-2) | 200 µL (Step-2) |
Step 2: Pipette 100 µL of this pre-diluted mixture, add 200 µL extracting solution, and vortex to obtain final test solution.
Extensively validated across over 50 fresh fruit and vegetable commodities:
STEP 1 Extraction Cut fresh sample into 0.5 cm pieces. Weigh 2.0 ± 0.1 g into sampling bottle, add 3 mL extracting solution, shake 1 min, let stand 1 min. (Centrifuge if cloudy). | STEP 2 Matrix Dilution Pipette X µL supernatant into a 2 mL tube, add Y µL extracting solution according to the matrix guide, and vortex 10 seconds to mix thoroughly. | STEP 3 Microwell & Load Ensure 20–40°C environment. Pipette 100 µL test solution into microwell, mix 3–5 times, stand 3 min. Transfer all to cassette sample well. | STEP 4 Read at 5–8 Mins Interpret visual lines strictly at 5–8 minutes. Visual readouts become strictly invalid after 8 minutes due to late chemical shifts. |
Negative (-): Both Control (C) line and Test (T) line develop strong, distinct red bands. Difenoconazole residue is safely below the targeted matrix LOD limit.
Positive (+): Control (C) line develops normally, but Test (T) line is visibly lighter or fails to appear → Difenoconazole residue ≥ targeted matrix LOD limit.
Invalid: Control (C) line fails to show any visible color band. Re-testing with a new cassette is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Matrix Dilution Accuracy: Strictly execute the X & Y dilution ratio corresponding to the commodity type to calibrate reading against exact regulatory MRL cutoffs.
Strict Time Window: Read visual test results strictly between 5–8 minutes after loading the cassette; results observed after 8 minutes are invalid.
Kit Components Included: 10 Test cassettes, 10 Microwells, 10 Centrifuge tubes (2 mL), Extracting Solution (70 mL) x1, 30 mL Sampling bottles (x2), User Manual.
Required Equipment (Not Provided): Scissors, electronic balance (2.0 ± 0.1 g precision), micropipettes (X & Y µL, 100 µL), vortexer, centrifuge (optional, for turbid extracts), and timer.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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