The FSTest Auramine O Rapid Test Kit (Basic Yellow 2) is a competitive inhibition lateral flow immunochromatographic assay engineered for rapid qualitative screening of illegal Auramine O dye residues in durian.

Auramine O is a toxic industrial diarylmethane dye strictly prohibited in food due to its carcinogenic and mutagenic risks. Offering an on-site detection threshold of 500 µg/kg (0.5 mg/kg) with clear visual results in only 8 to 10 minutes, this test kit allows fresh fruit importers, wholesalers, customs quarantine agencies, and food safety labs to conduct fast, dependable screening without relying on complex analytical instruments.

2. Key Benefits

Tailored Matrix Optimization: Specifically engineered and validated for challenging fruit matrices (durian pulp and peel), effectively eliminating natural matrix color and texture interference.
High Regulatory Sensitivity (500 µg/kg LOD): Accurately captures illegal Auramine O dye at 500 µg/kg (0.5 ppm), fully satisfying food safety surveillance standards.
Fast Visual Readout in 8–10 Minutes: Enables rapid qualitative interpretation on-site, drastically speeding up port-of-entry quarantine inspections and wholesale dispatch.
Simplified Sample Preparation: Requires no organic solvent extraction or complex SPE clean-up steps; utilizes a safe, ready-to-use aqueous dilution buffer.
Ambient Storage & Extended Shelf Life: Sealed storage at 4–30°C in a dry place with an 18-month shelf life, eliminating cold-chain transport dependency.

3. Technical Specifications & LOD Spectrum

Product Name	Auramine O Rapid Test Kit (Colloidal Gold)
Catalog Number	24134
Target Analyte Compound	Auramine O (Basic Yellow 2 \| CAS: 2465-27-2 \| C₁₇H₂₂ClN₃ \| 303.83 g/mol)
Detection Principle	Competitive Lateral Flow Immunochromatography (Colloidal Gold)
Pack Size	20 tests / kit
Assay Run Time & Volume	8–10 minutes (results invalid after 10 minutes); 100 µL test supernatant
Storage & Shelf Life	18 months sealed at 4–30°C in a cool, dry place (Do not freeze)

Target Compound Analytical Profile & Limits of Detection (LOD)

Target Analyte Compound	CAS Registry Number	Molecular Formula	Limit of Detection (LOD)
Auramine O (Basic Yellow 2)	2465-27-2	C₁₇H₂₂ClN₃	500 µg/kg (0.5 mg/kg)

4. Applicable Sample Types

Specifically engineered for identifying illegal dyeing practices in tropical fruit commodities:

Fresh Durian Pulp Homogenate Durian Peel Surface Scrapes & Homogenate Tropical Fruit Packing Houses & Processing Stations Customs Port-of-Entry Quarantine Inspection Wholesale Fruit Markets & Retail Supply Chains

5. Test Procedure

STEP 1

Extraction

Homogenize representative sample (> 100 g). Weigh 2.0 ± 0.1 g into a 15 mL tube, add 3 mL Dilution Solution, and vortex vigorously for 2 minutes.

STEP 2

Centrifugation

Centrifuge the tube at 4000 rpm for 5 minutes. The clear supernatant serves as ready-to-test solution (avoid aspirating floating debris).

STEP 3

Microwell Incubation

Ensure 20–40°C environment. Pipette 100 µL supernatant into a microwell, mix up and down 3–5 times, and incubate undisturbed for 3 minutes.

STEP 4

Load & Read (8–10 Mins)

Transfer all mixed liquid from microwell into cassette sample well. Interpret visual lines strictly at 8–10 minutes. Results are invalid after 10 minutes.

Visual Results Determination Guide:

Negative (-): Both Test (T) line and Control (C) line appear clearly. T line color intensity is equal to or darker than C line → Auramine O is absent or below 500 µg/kg.
Positive (+): Control (C) line appears distinctly, while Test (T) line is invisible or visibly lighter than C line → Auramine O is present at or above 500 µg/kg.
Invalid: Control (C) line fails to appear, regardless of whether T line is visible. Re-test with a fresh cassette.

6. Performance & Crucial Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

Qualitative Nature: Engineered strictly for preliminary qualitative screening; positive samples should be confirmed using chromatography methods (HPLC / LC-MS).
Moisture Protection: Start testing within 1 hour after removing the cassette from the foil pouch to prevent atmospheric moisture absorption and strip failure.
Environmental Conditions: Maintain operating ambient temperature between 5–40°C and relative humidity between 30%–80%. Perform assays sheltered from direct wind.
Strict Time Window: Read visual test results strictly between 8–10 minutes after loading cassette; results observed after 10 minutes are invalid.

7. Included Components & Auxiliary Equipment

Kit Components Included: 20 Test Cassettes (individually sealed with desiccant), 20 Microwells (pre-coated reagent wells), 20 Centrifuge Tubes (15 mL), 1 bottle of Dilution Solution (70 mL), User Manual.
Required Equipment (Not Provided): Sample homogenizer/blender, analytical balance (0.1 g precision), vortex mixer, centrifuge (≥ 4000 rpm), micropipette (100 µL range) & tips, and timer.

Request Samples & Ordering Information

Protect your brand and maintain trade compliance against illegal fruit dyeing in tropical fruit logistics. Free evaluation sample packs are available for certified testing laboratories, fruit packing houses, and import quarantine facilities.

Email: service@fstestcorp.com | Website: www.fstestcorp.com