The FSTest Tolfenpyrad Rapid Test Kit (Colloidal Gold) works on the sandwich lateral flow immunochromatography principle. Its test cassette has a testing window (with invisible T/test and C/control zones before use) for observing the assay and reading results.
When treated sample is added to the sample well, the liquid flows laterally across the strip and reacts with pre-coated recombinant antigens. The T line’s color intensity is inversely related to the sample’s tolfenpyrad concentration. The C line always appears to confirm a valid test—by comparing T and C line intensity, the kit indicates if tolfenpyrad is present in the sample.
Core Strengths: Accuracy & Reliability at Its Core
Precision-Driven Design: Engineered for targeted tolfenpyrad residue detection, with strict adherence to operational protocols that minimize error—ensuring consistent, trustworthy results for every test.
Controlled Performance Conditions: The test cassette must be equilibrated to 20-40℃ and placed on a flat surface before use, a critical step that maintains detection accuracy by eliminating environmental interference .
Valid Result Guarantees: Results are clearly interpretable within 5-8 minutes, and any readings after 8 minutes are deemed invalid—this strict timing ensures only accurate, non-degraded results are used for decision-making .
Stable Storage for Consistent Performance: Sealed storage at 4-30℃ (no freezing) preserves kit integrity for 12 months, ensuring reliable detection performance from first use to shelf-life end .
Streamlined Operation for Error-Free Testing
Designed to reduce operational mistakes, the kit follows a clear, step-by-step process:
Sample Pretreatment: Use the recommended extracting solution—vortex/shake the sample mixture for 1 minute, stand for 1 minute, and centrifuge at 4000 rpm for 3 minutes if supernatant is unclear (clarification ensures no sample impurities affect test accuracy) .
Sample Loading: Precisely pipette 100μL of pretreated sample into the cassette’s sample well, then stand for 3 minutes to ensure proper reaction , .
Result Reading: Refer to provided examples for interpretation, with validity limited to 5-8 minutes—removing ambiguity and ensuring correct result assessment .
Key Considerations for Sustained Reliability
Storage Compliance: Always store sealed at 4-30℃ in a cool, dry place; freezing is strictly prohibited to avoid damaging kit components .
Operational Hygiene: After testing, keep the laboratory clean and maintain air circulation—preventing cross-contamination that could compromise future test accuracy .
Pouch Handling: Only remove the cassette from its aluminum foil pouch when ready to use; exposure to excess moisture may degrade performance.