Comprehensive Food Safety Detection Solution

The FSTest™ Rhodamine B Rapid Test Kit is an advanced diagnostic tool designed to meet the rigorous demands of the food safety industry, quality control laboratories, and regulatory bodies. By combining state-of-the-art purification techniques with rapid lateral flow technology, this kit provides a highly reliable, qualitative method for detecting illicit Rhodamine B dye in complex food matrices.

Core Product Features & Analytical Advantages

Innovative Dual-Technology Integration: The FSTest™ kit seamlessly combines solid phase extraction (SPE) for sample purification with a competitive lateral flow immunochromatographic assay for precise, visual detection.

Ultra-High Sensitivity: Ensure your testing fully complies with strict food safety standards with a low Limit of Detection (LOD) of 5 µg/kg.

Unmatched Specificity: Say goodbye to false positives. The kit exhibits exceptional targeted accuracy, yielding negative results even when testing complex samples containing 100 µg/L of other common dyes, including Sudan Red, Sunset Yellow, Amaranth, and Tartrazine.

Rapid & Actionable Results: In fast-paced testing environments, time is critical. Once the prepared sample solution is added to the test cassette, clear and final results can be interpreted in just 5 to 8 minutes.

Built-in Procedural Quality Control: To guarantee the integrity of every test, each cassette features a dedicated control line (C line). This line will always appear during a test, regardless of the presence of Rhodamine B, confirming that the assay is valid and the capillary flow has functioned correctly.

Detailed Operating Principle

Targeted Purification via SPE: During the initial sample preparation, the liquid sample passes through a dedicated BSPE column containing an adsorbent that selectively retains the target analyte.

Efficient Elution & Separation: Impurities are effectively removed using a provided washing solvent. Subsequently, the purified analyte is eluted with a small volume of solvent to achieve rapid separation and purification without signal loss.

Competitive Binding Detection: During detection, any Rhodamine B present in the sample competes with the antigen immobilized on the test line (T line) for binding to colloidal gold-labeled antibodies.

Visual Interpretation: Because it is a competitive assay, the color intensity of the T line is inversely proportional to the concentration of Rhodamine B in the sample. By visually comparing the color intensity of the T line with that of the C line, technicians can qualitatively determine the presence of the contaminant.

Comprehensive Kit Specifications & Storage

Basic Specification

Each kit is fully equipped to perform 10 individual qualitative tests.

Included Components

✅10 Test cassettes

✅10 Microwells

✅10 BSPE columns for precision sample purification

✅10 × 10mL Centrifuge tubes & 10 × 15mL Centrifuge tubes

✅Complete Reagent Suite: Extraction solution (50mL ×2), Reconstitution solution (8mL ×1), Washing solution (25mL ×1), Elution solution (8mL ×1)

✅User manual

Storage & Environmental Guidelines

Storage Conditions: The kit must be stored sealed at 1-30°C in a cool and dry place. Do not freeze the reagents or cassettes.

Shelf Life: Under proper storage conditions, the product maintains a shelf-life of 18 months.

Operating Environment: The experimental environment should maintain a humidity level of 30%-80% and a temperature of 5°C-40°C. To avoid flaws in results due to moisture, testing should be completed within 1 hr after the test cassette is taken out of the package.

Applicable Scenarios & Target Matrices

Rhodamine B (Molecular Formula: C28H31ClN2O3, CAS: 81-88-9) is an illicit synthetic chemical dye. Our rapid test kit is specifically intended to qualitatively test for Rhodamine B in the following matrices:

✅Chili powder

✅Chili paste

✅Tomato sauce

✅Chili oil