The FSTest TVB-N (Total Volatile Basic Nitrogen) Rapid Test Kit adopts an advanced chemical colorimetric assay for fast on-site screening of protein spoilage and freshness across livestock, poultry, and aquatic meat products.

TVB-N serves as an essential international biomarker for evaluating animal food freshness. Bacterial and enzymatic decomposition breaks down proteins into volatile alkaline nitrogenous compounds (such as ammonia and amines), reducing nutritional value and posing substantial consumer health risks. Featuring an ultra-fast 2-minute reaction time and a standard visual colorimetric card, this kit enables meat processors, distributors, supermarkets, and regulators to verify meat freshness on-site without bulky Kjeldahl distillation or titration apparatus.

2. Key Benefits

Rapid 2-Minute Color Reaction: Delivers instantaneous freshness determination within 2 minutes of reagent addition, drastically speeding up receiving dock throughput.
High Sensitivity & Clear Gradation: Calibrated with an LOD of 5 mg/100g and graded scale up to 30 mg/100g, cleanly separating compliant fresh meat (≤ 15 mg/100g) from deteriorating stock.
Commercial 100-Test Format: High-capacity pack size significantly reduces cost-per-test for industrial slaughterhouses, processing lines, and high-volume labs.
Flexible Field Workflow: Supports both simple gravity filtration (using supplied filter paper) and rapid centrifugation (4000 rpm for 2 min) depending on operational context.
Direct Regulatory Benchmarking: Visual scale aligns directly with international and national food hygiene safety limits (e.g., GB standards for fresh livestock, poultry, and bullfrog meat).

3. Technical Specifications & Freshness Gradation

Product Name	TVB-N (Total Volatile Basic Nitrogen) Rapid Test Kit
Catalog Number	24138
Target Biomarker	Total Volatile Basic Nitrogen (Ammonia, Dimethylamine, Trimethylamine)
Detection Methodology	Visual Chemical Colorimetric Assay
Pack Size	100 tests / kit
Reaction Run Time	2 minutes
Storage & Shelf Life	12 months sealed at 4–30°C in a cool, dry place

Gradation Scale & Regulatory Compliance Benchmarks

Target Range	Colorimetric Steps	Freshness Compliance Standard
5 – 30 mg/100g	5, 10, 15, 20, 25, 30 mg/100g	≤ 15 mg/100g (Fresh meat & frog standard)

4. Applicable Sample Types

Specifically validated for monitoring freshness and protein breakdown across animal products:

Livestock Meat (Pork, beef, mutton muscle tissue) Poultry Meat (Chicken, duck meat tissue) Aquatic Specialties (Bullfrog muscle meat) Slaughterhouses, Cold-Chain Logistics & Supermarkets

5. Test Procedure

STEP 1

Sample Prep

Minced meat paste (1.0 g, skin/fat-free) into a 15 mL tube. Add 10 mL pure water, shake well, stand 5 min. Add 0.5 mL Reagent A and 0.5 mL Reagent B; mix well.

STEP 2

Clarification

Filter mixture through provided filter paper into a sample cup to collect ≥ 1 mL clear filtrate (or centrifuge at 4000 rpm for 2 min and collect supernatant).

STEP 3

Color Reaction

Pipette 200 µL filtrate into 2 mL tube. Add 1.8 mL pure water, 1 drop Reagent C, and 1 drop Reagent D. Cap securely, shake thoroughly, and react for 2 minutes.

STEP 4

Top-Down Read

Place tube on a white background. Open cap and observe liquid from a top-down view. Match color against the standard TVB-N Colorimetric Card.

Freshness Quality & Compliance Determination Guide:

Fresh Meat Grade (≤ 15 mg/100g): Matches 5, 10, or 15 mg/100g color blocks → Fully compliant with national food safety standards; safe for distribution and consumption.
Sub-Fresh / Secondary Grade (15 – 25 mg/100g): Matches 20 or 25 mg/100g color blocks → Pronounced protein breakdown; immediate processing or sorting recommended.
Spoiled / Decomposed Meat (≥ 30 mg/100g): Matches 30 mg/100g block or darker → Exceeds safety thresholds; unfit for consumption or sale; consignments must be rejected.

6. Performance & Crucial Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

Accurate Visual Observation: Always open the tube and view vertically from the top down against a clean matte white background under natural or diffused white light.
Corrosive Reagent Safety (Reagent D): Reagent D is corrosive and irritating. Wear lab coat, chemical-resistant gloves, and eye protection; handle with care.
Centrifuge Tube Cleaning: The 10 included 15 mL centrifuge tubes are reusable after thorough cleaning with neutral detergent and distilled/purified water.
Confirmatory Testing: This kit is intended for rapid sorting. Suspicious or borderline consignments should be confirmed via official distillation methods (e.g., Kjeldahl distillation).

7. Included Components & Auxiliary Equipment

Kit Components Included: TVB-N Reagents A, B, C, D (1 bottle each), 15 mL Centrifuge tubes x10, 2 mL Centrifuge tubes (1 bag), Sample cups x100, Filter paper x100, Standard Colorimetric Card x1, User Manual.
Required Equipment (Not Provided): Meat grinder/homogenizer, analytical scale, micropipette (200 µL) & tips, purified/distilled water, timer, and centrifuge (optional, for rapid separation).

Request Samples & Ordering Information

Evaluate the FSTest TVB-N Rapid Test Kit in your meat receiving and quality assurance inspection workflows. High-throughput evaluation packs are available for certified testing laboratories, processors, and distribution centers.

Email: service@fstestcorp.com | Website: www.fstestcorp.com