10–13 mins Rapid Readout Time | 0.1 mg/kg Fish Cadmium (Cd) LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Heavy Metal Cd Rapid Test Strip (for seafood) is an immunochromatographic assay designed for the qualitative screening of cadmium (Cd) heavy metal contamination in seafood samples, specifically fish and shrimp.
Utilizing pre-coated antigens, the test strip provides rapid, visual, and reliable results directly observed by the naked eye without requiring complex laboratory instrumentation. With matrix-tailored pre-treatment protocols, this kit offers seafood processors, aquaculture farms, and food safety regulators a field-ready solution to enforce cadmium compliance limits.
Matrix-Specific Sensitivity: Optimized extraction protocols tailored for fish tissue (0.1 mg/kg) and shrimp tissue (0.5 mg/kg) samples.
Rapid & Visual Screening: Delivers clear visual results within 10–13 minutes after reaction incubation.
User-Friendly Assay Format: Features pre-portioned microwells containing lyophilized reagents to streamline on-site testing.
Clear Signal Interpretation: Color intensity on the T-line inversely corresponds to cadmium concentration for unambiguous qualitative analysis.
Convenient Room Temp Storage: Sealed and stored at 4–30°C in a cool, dry place with a full 12-month shelf life.
| Product Name | Heavy Metal Cd Rapid Test Strip (for seafood) |
|---|---|
| Detection Principle | Competitive Immunochromatographic Assay |
| Pack Size | 10 tests / kit |
| Assay Time & Sample Volume | 10–13 minutes (results invalid after 13 minutes); 120 µL test solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Target Sample Matrix | CAS Registry Number | LOD Threshold (mg/kg) |
|---|---|---|
| Fish Tissue (Fat removed) | 7440-43-9 | 0.1 mg/kg |
| Shrimp Tissue | 7440-43-9 | 0.5 mg/kg |
Designed for matrix-specific cadmium screening in seafood commodities:
STEP 1 Fish Pretreatment Homogenize 2g fat-free fish tissue in 15mL tube. Add 0.8mL Reagent A & 3mL Reagent B, mix 3 min, centrifuge at 4000 rpm (2 min). Mix 200µL supernatant + 30µL Chelating Agent + 15µL Reagent C. | STEP 2 Shrimp Pretreatment Homogenize 1g shrimp tissue in 15mL tube. Add 0.5mL Reagent A & 3mL Reagent B, mix 3 min, centrifuge at 4000 rpm (2 min). Mix 100µL supernatant + 10µL Chelating Agent + 1mL purified water + 10µL Reagent C. | STEP 3 Load & Incubate Pipette 120µL of prepared solution into microwell, mix thoroughly, incubate for 3 min. Insert test strip into microwell and start timing immediately. | STEP 4 Read at 10–13 Mins Interpret results at 10–13 minutes. Visual readouts become strictly invalid after 13 minutes. |
Negative (-): Both Control (C) line and Test (T) line appear. Cadmium concentration is below the LOD threshold.
Positive (+): Control (C) line appears, while Test (T) line does not appear or is noticeably lighter → Cadmium concentration ≥ LOD threshold.
Invalid: Control (C) line fails to appear, regardless of T line presence. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Matrix Pretreatment Strictness: Ensure fish tissue fat is fully removed before grinding; for shrimp tissue, strictly add 1mL purified water during Step 2.
Strict Time Windows: Read visual test results strictly between 10–13 minutes; results observed after 13 minutes are invalid.
Kit Components Included: 10 Test strips, 10 Microwells, Reagent A (10mL) x1, Reagent B (40mL) x1, Heavy Metal Cd Chelating Agent (0.5mL) x1, Reagent C (0.3mL) x1, 15mL Centrifuge tubes (x10), 1.5mL Centrifuge tubes (x10), User Manual.
Required Equipment (Not Provided): Grinder/homogenizer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, timer, and purified water (required for shrimp testing).