β-Lactams + Tetracyclines + Sulfonamides 3-in-1 Rapid Test Strip
Brand: FSTest
7 mins Rapid Readout Time | 2–4 µg/kg Strict SAS & β-Lactam LOD | 96 Tests Commercial Bulk Pack | 18 Months Long Shelf Life |
The FSTest 3-in-1 Rapid Test Strip is an industrial-grade multiplex screening tool engineered for the simultaneous, independent detection of three major high-risk veterinary drug residue categories in dairy products: Beta-Lactams, Tetracyclines, and Sulfonamides.
Antibiotic residues in dairy production present critical food safety challenges and are subject to strict regulatory Maximum Residue Limits (MRLs) globally. By consolidating three distinct biochemical screenings into a single, straightforward assay loop, this kit eliminates the necessity for complex multiple extraction protocols or separate ELISA microplate platforms, compressing essential multi-residue milk verification into a simplified, field-ready ambient workflow for dairy farms, milk collection stations, pasteurization plants, milk powder manufacturers, and quality control laboratories.
High-Speed Tri-Multiplex Screening: Maps out a comprehensive safety profile spanning three major contaminant groups in a single test sequence, providing clear visual results within just 7 minutes.
Ultra-High Sensitivity Thresholds: Calibrated to deliver highly sensitive qualitative screenings: Sulfonamides down to 2–4 µg/kg across 17 compounds, Tetracyclines down to 5 µg/kg, and Beta-Lactams down to 2–4 µg/kg baseline across 18 molecules.
Extensive Dairy Matrix Adaptability: Formulated for multi-matrix sample validation, providing reliable results for raw milk, pasteurized liquid milk, and reconstituted milk powder formulations.
Zero-Ambiguity Visual Layout: Features three separate invisible Test lines (T1: Sulfonamides, T2: Tetracyclines, T3: Beta-Lactams) paired with an integrated internal Control (C) line for clear reading without cross-line visual interference.
Heavy-Duty Commercial Specification: Supplied in a bulk 96-test pack (96 test strips and 96 dry-reagent precoated microwells) perfectly optimized for high-throughput batch auditing.
Institutional Shelf-Life Flexibility: Offers a full 18-month sealed storage lifespan (2–8°C) with an advanced ambient tolerance profile permitting room-temperature transport up to 30 days.
| Product Name | β-Lactams + Tetracyclines + Sulfonamides 3-in-1 Rapid Test Strip |
|---|---|
| Assay Mechanism | Competitive Multiplex Lateral Flow Immunochromatography |
| Pack Size | 96 Tests / Kit (96 Test Strips + 96 Precoated Microwells) |
| Test Time | 3 minutes microwell incubation + 7 minutes strip reaction |
| Storage & Shelf Life | 18 months at 2–8°C; permits room-temperature transport/storage up to 30 days |
| 1–2. Target Analyte Compound | CAS Registry Number | LOD Threshold (µg/kg) |
|---|---|---|
| Sulfamethazine (T1 Line) | 57-68-1 | 2–4 µg/kg |
| Sulfamonomethoxine (T1 Line) | 1220-83-3 | 2–4 µg/kg |
| Sulfadiazine (T1 Line) | 68-35-9 | 2–4 µg/kg |
| Sulfametoxydiazine / Sulfameter (T1 Line) | 651-06-9 | 2–4 µg/kg |
| Sulfamerazine (T1 Line) | 127-79-7 | 5 µg/kg |
| Sulfadimethoxine (T1 Line) | 122-11-2 | 10 µg/kg |
| Sulfaquinoxaline (T1 Line) | 59-40-5 | 10 µg/kg |
| Sulfchlorpyridazine (T1 Line) | 80-32-0 | 10 µg/kg |
| Sulfamethizole (T1 Line) | 144-82-1 | 20 µg/kg |
| Sulfisoxazole (T1 Line) | 127-69-5 | 20 µg/kg |
| Sulfamethoxypyridazine (T1 Line) | 80-35-3 | 20 µg/kg |
| Sulfafurazole (T1 Line) | 127-69-5 | 100 µg/kg |
| Sulfalene (T1 Line) | 152-47-6 | 100 µg/kg |
| Sulfamethoxazole (T1 Line) | 723-46-6 | 100 µg/kg |
| Sulfadoxine (T1 Line) | 2447-57-6 | 100 µg/kg |
| Sulfabenzamide (T1 Line) | 127-71-9 | 200 µg/kg |
| Sulfathiazole (T1 Line) | 72-14-0 | 200 µg/kg |
| Tetracycline (T2 Line) | 60-54-8 | 5 µg/kg |
| Chlortetracycline (T2 Line) | 57-62-5 | 5 µg/kg |
| Oxytetracycline (T2 Line) | 79-57-2 | 5 µg/kg |
| Doxycycline (T2 Line) | 100929-47-3 | 5 µg/kg |
| 3. Beta-Lactams (T3 Line) Compound | CAS Registry Number | LOD Threshold (µg/kg) |
|---|---|---|
| Penicillin G Sodium Salt | 69-57-8 | 2–4 µg/kg |
| Penicillin-V | 87-08-1 | 2–4 µg/kg |
| Piperacillin | 61477-96-1 | 2–4 µg/kg |
| Ampicillin Trihydrate | 7177-48-2 | 2–4 µg/kg |
| Amoxicillin Trihydrate | 61336-70-7 | 2–4 µg/kg |
| Oxacillin | 66-79-5 | 2–4 µg/kg |
| Cloxacillin | 61-72-3 | 2–4 µg/kg |
| Cefoperazone | 62893-19-0 | 2–4 µg/kg |
| Cephalonium | 5575-21-3 | 2–4 µg/kg |
| Cefacetrile | 10206-21-0 | 8 µg/kg |
| Nafcillin | 147-52-4 | 10 µg/kg |
| Cefapirin | 21593-23-7 | 10 µg/kg |
| Cephalothin | 153-61-7 | 10 µg/kg |
| Cefquinome sulfate | 118443-89-3 | 20 µg/kg |
| Ceftiofur | 80370-57-6 | 50 µg/kg |
| Ceftriaxone | 73384-59-5 | 100 µg/kg |
| Cefazolin | 25953-19-9 | 100 µg/kg |
| Cephalexin monohydrate | 23325-78-2 | 1000 µg/kg |
Extensively validated for multi-matrix dairy applications across the supply chain:
STEP 1 Sample Prep Equilibrate milk to 20–40°C. For powder, mix 1 g powder in 9 mL distilled water. Take out strips/wells and re-cap tube immediately. | STEP 2 Mix & Incubate Pipette 150 µL milk into precoated microwell. Mix thoroughly by repeated pipetting and incubate for 3 min (at room temp if ≥ 20°C, or set incubator at 40°C). | STEP 3 Insert Strip Insert test strip with loading end facing down into microwell. Start timing immediately for lateral flow reaction. | STEP 4 Read at 7 Mins Read visual lines strictly at 7 minutes. Results become invalid after 10 minutes due to post-reaction chemical shifts. |
Negative (-): Control (C) and all three Test lines (T1, T2, T3) show strong, distinct red bands matching or darker than C-line. (All 3 groups below cutoff).
T1 Positive (+): T1 line is visibly lighter than C-line or disappears completely; Sulfonamides residue ≥ threshold limit.
T2 Positive (+): T2 line is visibly lighter than C-line or disappears; Tetracyclines residue ≥ 5 µg/kg.
T3 Positive (+): T3 line is visibly lighter than C-line or disappears; Beta-Lactams residue ≥ threshold limit.
Multiplex Positive (+): Multiple faded or absent T-lines indicate co-existing multi-drug contamination.
Invalid: Control (C) line fails to develop a visible color band. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for rapid qualitative prescreening and preliminary field audits. Confirmatory laboratory testing is recommended if necessary.
Air Exposure Window: Initiate test within 30 minutes after pulling strips from protective container to prevent moisture damage.
Meticulous Pipetting: Thoroughly resuspend dry microwell reagents via repeated fluidic pipetting; improper mixing impairs line color development.
Environmental Limits: Maintain operating relative humidity at 30%–80%. Avoid wind currents and testing above 40°C. Do not touch central white membrane area.
Dairy Portable Incubator Block (40°C): Constant temperature incubator block for standardized testing when ambient temperature is below 20°C.
Handheld Optical Test Strip Reader: Optional digital strip reader for objective line intensity recording and cloud data upload.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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