Sulfonamides + Tetracyclines Rapid Test Strip
Brand: FSTest
7 mins Rapid Readout Time | 2–4 µg/kg Strict SAS Sensitivity | 96 Tests Commercial Pack | 18 Months Long Shelf Life |
The FSTest Sulfonamides + Tetracyclines Rapid Test Strip is a competitive immunochromatographic assay engineered for the simultaneous, qualitative detection of sulfonamide and tetracycline residues in raw milk, pasteurized milk, and reconstituted milk powder.
Utilizing a dual-line test strip pre-coated with recombinant antigens, the assay enables simple, rapid, and visual multi-residue screening without requiring complex analytical instrumentation. Within just 7 minutes, it produces clear visual results to enforce compliance with international Maximum Residue Limits (MRLs) at raw milk intake stations and processing plants.
Dual Antibiotic Target Screening: Simultaneously detects Tetracyclines (T1 line) and Sulfonamides (T2 line) residues in a single strip assay.
Broad Spectrum Profile: Validates compliance across a wide range of 18 sulfonamide derivatives and 4 tetracycline class antibiotics.
Rapid On-Site Workflow: Requires no complex sample preparation; yields clear visual results in just 7 minutes.
Flexible Operating Conditions: Supports room temperature assay execution (≥20°C) or controlled incubator heating at 40°C.
Long Storage Life: Stored at 2–8°C for up to 18 months; permits room-temperature transport and field deployment for up to 30 days.
| Product Name | Sulfonamides + Tetracyclines Rapid Test Strip |
|---|---|
| Target Analytes | Tetracyclines (T1 Line) & Sulfonamides (T2 Line) |
| Detection Principle | Competitive Immunochromatographic Assay |
| Pack Size | 96 tests / kit (96 Test Strips + 96 Precoated Microwells) |
| Assay Time & Sample Volume | 7 minutes (results invalid after 10 minutes); 150 µL sample volume |
| Storage & Shelf Life | 18 months stored at 2–8°C; transportable at room temp up to 30 days (Do not freeze) |
| 1. Sulfonamides (T2 Line) Compound | Target Class | LOD Threshold (µg/kg) |
|---|---|---|
| Sulfamethazine | Sulfonamides | 2–4 µg/kg |
| Sulfamonomethoxine | Sulfonamides | 2–4 µg/kg |
| Sulfametoxydiazine | Sulfonamides | 2–4 µg/kg |
| Sulfamerazine | Sulfonamides | 5 µg/kg |
| Sulfadimethoxine | Sulfonamides | 10 µg/kg |
| Sulfaquinoxaline | Sulfonamides | 10 µg/kg |
| Sulfadiazine | Sulfonamides | 10 µg/kg |
| Sulfachloropyridazine | Sulfonamides | 10 µg/kg |
| Sulfamethizole | Sulfonamides | 20 µg/kg |
| Sulfisoxazole | Sulfonamides | 20 µg/kg |
| Sulfamethoxypyridazine | Sulfonamides | 20 µg/kg |
| Sulfafurazole | Sulfonamides | 100 µg/kg |
| Sulfalene | Sulfonamides | 100 µg/kg |
| Sulfamethoxazole | Sulfonamides | 100 µg/kg |
| Sulfadoxine | Sulfonamides | 100 µg/kg |
| Sulfabenzamide | Sulfonamides | 200 µg/kg |
| Sulfathiazole | Sulfonamides | 200 µg/kg |
| Sulfapyridine | Sulfonamides | 500 µg/kg |
| 2. Tetracyclines (T1 Line) Compound | Target Class | LOD Threshold (µg/kg) |
|---|---|---|
| Tetracycline | Tetracyclines | 5 µg/kg |
| Chlortetracycline | Tetracyclines | 5 µg/kg |
| Oxytetracycline | Tetracyclines | 5 µg/kg |
| Doxycycline | Tetracyclines | 5 µg/kg |
Designed for quality verification across liquid and powder dairy commodities:
STEP 1 Preparation Equilibrate milk to 20–40°C. For milk powder, mix 1 g powder with 9 mL distilled water until completely dissolved. | STEP 2 Sample Incubation Place microwells in incubator (or room temp if >20°C). Pipette 150 µL milk into microwell, mix thoroughly by pipetting, and incubate for 3 min. | STEP 3 Assay Execution Insert test strip into the microwell and start timing immediately for capillary lateral flow. | STEP 4 Read at 7 Mins Read visual lines strictly at 7 minutes. Results become invalid after 10 minutes due to post-reaction chemical shifts. |
Negative (-): Control line (C), T1 line (Tetracyclines), and T2 line (Sulfonamides) all appear dark and distinct. (Both classes below cutoff).
T1 Positive (+): T1 line is significantly lighter than C line or invisible → Tetracyclines residue detected.
T2 Positive (+): T2 line is significantly lighter than C line or invisible → Sulfonamides residue detected.
T1 & T2 Positive (+): Both T1 and T2 lines are lighter than C line or invisible → Both target classes detected.
Invalid: Control (C) line fails to appear, regardless of T1 or T2 line presence. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for rapid qualitative prescreening and preliminary field audits. Confirmatory laboratory testing is recommended if necessary.
Air Exposure Limit: Use strips within 30 minutes after pulling from vial to prevent dampness and loss of reactivity.
Meticulous Pipetting: Thoroughly resuspend dry microwell reagents via repeated fluidic pipetting; improper mixing impairs line color development.
Environmental Limits: Maintain operating relative humidity at 30%–80%. Avoid direct wind currents and testing above 40°C.
Kit Components Included: 96 Test strips, 96 Precoated Microwells, and 1 User Manual.
Required Auxiliary Equipment (Not Provided): Micropipettes (150 µL), timer, and incubator (recommended if ambient temperature <20°C).
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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