β-Lactams + Tetracyclines + Sulfonamides + Aflatoxin M1 4-in-1 Rapid Test Strip
Cat NO.: 11113 | Brand: FSTest
7 mins Rapid Readout Time | 0.05 µg/kg Ultra-Strict AFM1 LOD | 96 Tests Commercial Bulk Pack | 24 Months Long Shelf Life |
The FSTest β-Lactams + Tetracyclines + Sulfonamides + Aflatoxin M1 4-in-1 Rapid Test Strip (CatNO: 11113) is an advanced, industrial-grade multiplex screening tool engineered for the simultaneous, independent detection of four major high-risk chemical contaminant categories in dairy products: Beta-Lactams, Tetracyclines, Sulfonamides, and Aflatoxin M1.
Antibiotic residues and dangerous mycotoxins pose severe public health concerns and are subject to stringent Maximum Residue Limits (MRLs) globally. By consolidating four distinct biochemical screenings into a single, straightforward assay loop, this kit eliminates the necessity for complex multiple extraction protocols or separate ELISA microplate platforms. It compresses essential multi-residue milk verification into a simplified, field-ready ambient workflow for dairy farms, raw milk collection stations, pasteurization plants, and QA laboratories.
High-Speed Quad-Multiplex Screening: Maps out a comprehensive safety profile spanning four major contaminant groups in a single test sequence with visual results in just 7 minutes.
Ultra-High Sensitivity Thresholds: Calibrated to meet or surpass rigid regulatory cutoffs: Aflatoxin M1 down to 0.05 µg/kg, Sulfonamides down to 2–4 µg/kg, Tetracyclines down to 5 µg/kg, and Beta-Lactams down to 2–4 µg/kg.
Extensive Dairy Matrix Adaptability: Formulated for multi-matrix sample validation, providing reliable results for raw milk, pasteurized liquid milk, and reconstituted milk powder.
Zero-Ambiguity Visual Layout: Features four separate invisible Test lines (T1: Aflatoxin M1, T2: Sulfonamides, T3: Tetracyclines, T4: Beta-Lactams) paired with an integrated internal Control (C) line for clear reading.
Heavy-Duty Commercial Specification: Supplied in a bulk 96-test pack (96 test strips and 96 dry-reagent precoated microwells) optimized for high-throughput batch auditing.
Institutional Shelf-Life Flexibility: Offers a full 24-month sealed storage lifespan (2–8°C) with an advanced ambient tolerance profile permitting room-temperature transport up to 30 days.
| Product Name | β-Lactams + Tetracyclines + Sulfonamides + Aflatoxin M1 4-in-1 Rapid Test Strip |
|---|---|
| Catalog Number (CatNO) | 11113 |
| Assay Mechanism | Competitive Multiplex Lateral Flow Immunochromatography |
| Pack Size | 96 Tests / Kit (96 Test Strips + 96 Precoated Microwells) |
| Test Time | 3 minutes microwell incubation + 7 minutes strip reaction |
| Storage & Shelf Life | 24 months at 2–8°C; permits room-temperature transport/storage up to 30 days |
| Target Group (Test Line) | Target Analyte Compound | CAS Number | Formula / Mol. Weight | LOD Cutoff |
|---|---|---|---|---|
| 1. Aflatoxin M1 (T1 Line) | Aflatoxin M1 | 6795-23-9 | - | 0.05 µg/kg |
| 2. Tetracyclines (T3 Line) | Tetracycline | 60-54-8 | C₄H₁₄N₈O₄S₃ | 5 µg/kg |
| Chlortetracycline | 57-62-5 | C₁₆H₁₉N₃O₅S | 5 µg/kg | |
| Oxytetracycline | 79-57-2 | C₂₃H₂₅N₆O₉S₃ | 5 µg/kg | |
| Doxycycline hydrochloride | 100929-47-3 | C₁₉H₁₇N₅O₇S | 5 µg/kg |
| 3. Sulfonamides (T2 Line) Compound | CAS Registry Number | Molecular Weight | LOD Threshold (µg/kg) |
|---|---|---|---|
| Sulfamethazine | 57-68-1 | 278.33 g/mol | 2–4 µg/kg |
| Sulfamonomethoxine | 1220-83-3 | 280.30 g/mol | 2–4 µg/kg |
| Sulfadimethoxine | 122-11-2 | 310.33 g/mol | 2–4 µg/kg |
| Sulfametoxydiazine / Sulfameter | 651-06-9 | 280.30 g/mol | 2–4 µg/kg |
| Sulfamerazine | 127-79-7 | 264.30 g/mol | 5 µg/kg |
| Sulfafurazole | 127-69-5 | 267.30 g/mol | 10 µg/kg |
| Sulfaquinoxaline | 59-40-5 | 300.34 g/mol | 10 µg/kg |
| Sulfadiazine | 68-35-9 | 250.28 g/mol | 10 µg/kg |
| Sulfachloropyridazine | 80-32-0 | 284.72 g/mol | 10 µg/kg |
| SulfMethizole | 144-82-1 | 270.33 g/mol | 20 µg/kg |
| Sulfisoxazole | 127-69-5 | 267.30 g/mol | 20 µg/kg |
| Sulfamethoxypyridazine | 80-35-3 | 280.30 g/mol | 20 µg/kg |
| Sulfabenzamide | 127-71-9 | 276.31 g/mol | 100 µg/kg |
| Sulfalene | 152-47-6 | 280.30 g/mol | 100 µg/kg |
| Sulfamethoxazole | 723-46-6 | 253.29 g/mol | 100 µg/kg |
| Sulfadoxine | 2447-57-6 | 310.33 g/mol | 100 µg/kg |
| Sulfapyridine | 144-83-2 | 249.29 g/mol | 500 µg/kg |
| 4. Beta-Lactams (T4 Line) Compound | CAS Registry Number | LOD Threshold (µg/kg) |
|---|---|---|
| Penicillin G Sodium Salt | 69-57-8 | 2–4 µg/kg |
| Penicillin-V | 87-08-1 | 2–4 µg/kg |
| Piperacillin | 61477-96-1 | 2–4 µg/kg |
| Ampicillin Trihydrate | 7177-48-2 | 2–4 µg/kg |
| Amoxicillin Trihydrate | 61336-70-7 | 2–4 µg/kg |
| Oxacillin | 66-79-5 | 2–4 µg/kg |
| Cloxacillin | 61-72-3 | 2–4 µg/kg |
| Cefoperazone | 62893-19-0 | 2–4 µg/kg |
| Cephalonium | 5575-21-3 | 2–4 µg/kg |
| Cefacetrile | 10206-21-0 | 8 µg/kg |
| Nafcillin | 147-52-4 | 10 µg/kg |
| Cefapirin | 21593-23-7 | 10 µg/kg |
| Cephalothin | 153-61-7 | 10 µg/kg |
| Cefquinome sulfate | 118443-89-3 | 20 µg/kg |
| Ceftiofur | 80370-57-6 | 50 µg/kg |
| Ceftriaxone | 73384-59-5 | 100 µg/kg |
| Cefazolin | 25953-19-9 | 100 µg/kg |
| Cephalexin monohydrate | 23325-78-2 | 1000 µg/kg |
Extensively validated for multi-matrix dairy applications across the supply chain:
STEP 1 Sample Prep Equilibrate milk to 20–40°C. For powder, mix 1 g powder in 9 mL distilled water. Take out strips/wells and re-cap vial immediately. | STEP 2 Mix & Incubate Pipette 150 µL milk into precoated microwell. Mix thoroughly by repeated pipetting and incubate for 3 min (at room temp if ≥ 20°C, or set incubator at 40°C). | STEP 3 Insert Strip Insert test strip with loading end facing down into microwell. Start timing immediately for lateral flow reaction. | STEP 4 Read at 7 Mins Read visual lines strictly at 7 minutes. Results become invalid after 10 minutes due to post-reaction chemical shifts. |
Negative (-): Control (C) and all four Test lines (T1, T2, T3, T4) show strong, distinct red bands matching or darker than C-line. (All 4 groups below cutoff).
T1 Positive (+): T1 line is visibly lighter than C-line or disappears completely → Aflatoxin M1 residue ≥ 0.05 µg/kg.
T2 Positive (+): T2 line is visibly lighter than C-line or disappears → Sulfonamides residue ≥ threshold limit.
T3 Positive (+): T3 line is visibly lighter than C-line or disappears → Tetracyclines residue ≥ 5 µg/kg.
T4 Positive (+): T4 line is visibly lighter than C-line or disappears → Beta-Lactams residue ≥ threshold limit.
Multiplex Positive (+): Multiple faded or absent T-lines indicate co-existing multi-contaminant residues.
Invalid: Control (C) line fails to develop a visible color band. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for rapid qualitative prescreening and preliminary field audits. Confirmatory laboratory testing is recommended if necessary.
Air Exposure Window: Initiate test within 30 minutes after pulling strips from protective vial to prevent moisture damage.
Meticulous Pipetting: Thoroughly resuspend dry microwell reagents via repeated fluidic pipetting; improper mixing impairs line color development.
Environmental Limits: Maintain operating relative humidity at 30%–80%. Avoid wind currents and testing above 40°C. Do not touch central white membrane area.
Dairy Portable Incubator Block (40°C): Constant temperature incubator block for standardized testing when ambient temperature is below 20°C.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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