Histamine Rapid Test Strip
Brand: FSTest
5 mins Rapid Readout Time | 30 mg/kg High Sensitivity (LOD) | 25 Tests Complete Kit Pack | 18 Months Long Shelf Life (4–30°C) |
The FSTest Histamine Rapid Test Strip is a competitive lateral flow immunochromatographic assay designed for the rapid qualitative screening of histamine levels in marine fish, freshwater fish, and their processed products.
Histamine is a toxic biogenic amine produced during bacterial spoilage of scombroid and other fish species, posing severe food poisoning risks. The test strip features pre-coated recombinant antigens that react with target analytes, where the intensity of color on the Test (T) line corresponds inversely to the histamine concentration in the sample. Visual screening results are generated in just 5 minutes without requiring complex laboratory instrumentation, making it an ideal field tool for seafood processing plants, dockside inspections, and quality control laboratories.
Rapid 5-Minute Screening: Delivers actionable visual results in 5 minutes to accelerate dockside raw material release and processing audits.
High Sensitivity Cutoff: Reliably detects histamine contamination down to 30 mg/kg (ppm), meeting strict seafood safety guidelines.
Broad Aquatic Matrix Adaptability: Suitable for both marine fish (tuna, mackerel, etc.) and freshwater fish products.
Complete Ready-to-Use Kit: Packaged with extraction solutions, diluents, microwells, and centrifuge tubes for seamless field testing.
Convenient Room Temp Storage: Offers an 18-month shelf life stored at 4–30°C without requiring refrigeration.
| Product Name | Histamine Rapid Test Strip |
|---|---|
| Detection Principle | Competitive Lateral Flow Immunochromatography Assay |
| Pack Size | 25 tests / kit |
| Test Time & Sample Volume | 5 minutes (results invalid after 7 minutes); 150 µL test solution |
| Storage & Shelf Life | 18 months at 4–30°C in a cool, dry place (Do not freeze) |
| Target Analyte Compound | CAS Registry Number | LOD Threshold (mg/kg) |
|---|---|---|
| Histamine (C₅H₉N₃, 111.15 g/mol) | 51-45-6 | 30 mg/kg |
Intended for testing histamine levels across various fish commodities:
STEP 1 Sample Prep Dilute 10x extraction solution 1:9 with purified water. Mash 100g sample. Weigh 1g into 15mL tube, add 10mL diluted extract, shake for 3 min. | STEP 2 Dilute Supernatant Centrifuge at 4000rpm for 1 min. Take 0.1mL supernatant, add 0.9mL histamine diluent, and mix well for 30 seconds. | STEP 3 Load & Incubate Ensure test strips/samples are at 20–40°C. Pipette 150 µL solution into microwell, insert test strip, and start timing. | STEP 4 Read at 5 Mins Interpret results in 5 minutes. Visual readouts become strictly invalid after 7 minutes. |
Negative (-): Both Control (C) line and Test (T) line show strong, distinct bands. Histamine concentration is below 30 mg/kg.
Positive (+): Control (C) line appears, while Test (T) line is significantly lighter or invisible → Histamine concentration ≥ 30 mg/kg.
Invalid: Control (C) line fails to appear. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Designed exclusively for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Air Exposure Window: Once removed from the container pouch, carry out the assay within 1 hour to prevent moisture damage.
Environmental Limits: Maintain relative humidity between 30%–80%. Avoid wind currents and temperatures above 40°C or below 5°C.
Operational Restrictions: Single-use only. Do not touch the white membrane area in the middle of the test strip.
Kit Components Included: 25 Test strips, 10x Histamine extracting solution (30 mL), Histamine diluent (30 mL), 15 mL centrifuge tubes (x25), 2 mL centrifuge tubes (x25), Blank microwells (x25), Microplate, User manual.
Required Equipment (Not Provided): Purified water, homogenizer/masher, scale, centrifuge (4000 rpm), micropipette, and timer.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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