5–8 mins Rapid Readout Time | 0.5 mg/kg Strict Lead (Pb) LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Heavy Metal Pb Rapid Test Strip (for seafood) is an immunochromatographic assay designed for the qualitative screening of lead (Pb) contamination in seafood samples.
The test strip utilizes pre-coated antigens to perform a lateral flow assay, providing rapid, visual, and reliable results to determine the presence of lead directly observed by the naked eye without requiring complex laboratory instrumentation. Tailored specifically for complex seafood matrices, this assay offers seafood processors, aquaculture managers, and market regulators a dependable, on-site heavy metal safety screening solution.
Seafood-Specific Optimization: Tailored sample extraction and chelating process for accurate heavy metal lead screening in seafood matrices.
Rapid On-Site Detection: Streamlined testing workflow delivering visual results in just 5–8 minutes after incubation.
User-Friendly Assay Format: Includes pre-portioned microwells with lyophilized reagents to simplify hands-on operation.
Clear Visual Interpretation: T-line color intensity inversely corresponds to the lead concentration for unambiguous qualitative analysis.
Convenient Room Temp Storage: Offers a full 12-month shelf life when sealed and stored at 4–30°C in a cool, dry place.
| Product Name | Heavy Metal Pb Rapid Test Strip (for seafood) |
|---|---|
| Detection Principle | Competitive Immunochromatographic Assay |
| Pack Size | 10 tests / kit |
| Assay Time & Sample Volume | 5–8 minutes (results invalid after 8 minutes); 120 µL test solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Target Analyte Compound | CAS Registry Number | LOD Threshold (mg/kg) |
|---|---|---|
| Lead (Pb Heavy Metal, 207.2 g/mol) | 7439-92-1 | 0.5 mg/kg |
Optimized for qualitative screening of heavy metal lead in seafood commodities:
STEP 1 Extraction Homogenize tissue. Weigh 1g into 15mL tube, add 0.8mL Reagent A & 3mL Reagent B. Mix fully for 3 min, centrifuge at 4000 rpm for 2 min. | STEP 2 Chelating Prep Pipette 10µL supernatant into 2mL tube. Add 10µL Chelating Agent & 1.5mL purified water, mix thoroughly. Add 10µL Reagent C & mix. | STEP 3 Load & Incubate Pipette 120µL test solution into microwell, mix thoroughly, incubate for 3 min. Insert test strip into microwell & start timing. | STEP 4 Read at 5–8 Mins Interpret visual lines at 5–8 minutes. Visual results become strictly invalid after 8 minutes. |
Negative (-): Both Control (C) line and Test (T) line appear distinctly. Lead concentration is below 0.5 mg/kg.
Positive (+): Control (C) line appears, while Test (T) line does not appear or is significantly fainter → Lead concentration ≥ 0.5 mg/kg.
Invalid: Control (C) line fails to appear, regardless of T line presence. Re-testing with a new strip is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Critical Chelating Step: Thorough stirring during Step 2 (adding Heavy Metal Pb Chelating Agent and purified water) is critical for complete chelating reaction.
Strict Time Windows: Read visual test results within 5–8 minutes; results observed after 8 minutes are invalid.
Kit Components Included: 10 Test strips, 10 Microwells, Reagent A (6mL) x1, Reagent B (40mL) x1, Heavy Metal Pb Chelating Agent (0.2mL) x1, Reagent C (0.2mL) x1, 15mL Centrifuge tubes (x10), 2mL Centrifuge tubes (x10), User Manual.
Required Equipment (Not Provided): Purified water, grinder/homogenizer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, and timer.