Application: Designed for quick and accurate detection of Bata-Lactams, Tetracyclines antibiotic residues in raw milk, pasteurized milk, milk powder，goat's milk, camel's milk, and buffalo milk.

β-Lactams + Tetracyclines Rapid Test Strip

β-Lactams + Tetracyclines Rapid Test Strip

Dual-Target Competitive Immunochromatographic Assay for Dairy Antibiotic Screening

2-IN-1 MULTIPLEX TEST
Brand: FSTest

7 mins

Rapid Readout Time

2–4 µg/kg

Strict β-Lactam LOD

96 Tests

Commercial Pack

18 Months

Long Shelf Life

1. Product Overview

The FSTest® β-Lactams + Tetracyclines Rapid Test Strip is an immunochromatographic assay designed for the simultaneous qualitative detection of β-Lactam and Tetracycline antibiotic residues in dairy products, including raw milk, pasteurized milk, and milk powder.

Utilizing a dual-line lateral flow test strip format, the assay features pre-coated recombinant antigens that react with target residues, providing visual screening results directly observed by the naked eye without requiring complex laboratory instrumentation. Within just 7 minutes, it produces clear visual results to enforce compliance with international Maximum Residue Limits (MRLs) at raw milk intake stations and processing plants.

2. Key Benefits

Dual Antibiotic Target Screening: Simultaneously detects Tetracyclines (T1 line) and β-Lactams (T2 line) residues in a single strip assay.
High Sensitivity & Broad Spectrum: Detects a wide variety of Penicillins, Cephalosporins, and Tetracyclines down to low ppb levels.
Rapid On-Site Workflow: Direct sample application with visual result interpretation in just 7 minutes.
Flexible Incubation Options: Suitable for room temperature testing (≥20°C) or controlled incubator-assisted testing at 40°C.
Long Storage Life: Stored at 2–8°C for up to 18 months; permits room-temperature transport and field deployment for up to 30 days.

3. Technical Specifications & LOD Spectrum

Product Name	β-Lactams + Tetracyclines Rapid Test Strip
Target Analytes	Tetracyclines (T1 Line) & β-Lactams (T2 Line)
Detection Principle	Competitive Immunochromatographic Assay
Pack Size	96 tests / kit (96 Test Strips + 96 Precoated Microwells)
Assay Time & Sample Volume	7 minutes (results invalid after 10 minutes); 150 µL sample volume
Storage & Shelf Life	18 months stored at 2–8°C; transportable at room temp up to 30 days (Do not freeze)

Detailed Analyte Sensitivity Spectrum (Limit of Detection / LOD)

1. β-Lactams (T2 Line) Compound	Target Class	LOD Threshold (µg/kg)
Penicillin G	β-Lactams	2–4 µg/kg
Penicillin V	β-Lactams	4 µg/kg
Piperacillin	β-Lactams	4 µg/kg
Ampicillin	β-Lactams	4 µg/kg
Amoxicillin	β-Lactams	4 µg/kg
Oxacillin	β-Lactams	4 µg/kg
Cloxacillin	β-Lactams	4 µg/kg
Cefoperazone	β-Lactams	4 µg/kg
Cefalonium	β-Lactams	4 µg/kg
Cefacetrile	β-Lactams	8 µg/kg
Nafcillin	β-Lactams	10 µg/kg
Cefapirin	β-Lactams	10 µg/kg
Cephalothin	β-Lactams	10 µg/kg
Cefquinome	β-Lactams	20 µg/kg
Ceftiofur	β-Lactams	50 µg/kg
Ceftriaxone	β-Lactams	100 µg/kg
Cefazolin	β-Lactams	100 µg/kg
Cefalexin	β-Lactams	1000 µg/kg

2. Tetracyclines (T1 Line) Compound	Target Class	LOD Threshold (µg/kg)
Tetracycline	Tetracyclines	5 µg/kg
Chlortetracycline	Tetracyclines	5 µg/kg
Oxytetracycline	Tetracyclines	5 µg/kg
Doxycycline	Tetracyclines	5 µg/kg

4. Applicable Sample Types

Designed for quality verification across liquid and powder dairy commodities:

Raw Bovine Cow Milk Pasteurized & UHT Commercial Liquid Milk Reconstituted Milk Powder (1:9 water ratio) Dairy Farm Bulk Tanks & Intake Stations

5. Test Procedure

STEP 1

Preparation

Equilibrate milk to 20–40°C. For milk powder, mix 1 g powder with 9 mL distilled water until completely dissolved.

STEP 2

Sample Incubation

Place microwells in incubator (or room temp if >20°C). Pipette 150 µL milk into microwell, mix thoroughly by pipetting, and incubate for 3 min.

STEP 3

Assay Execution

Insert test strip into the microwell and start timing immediately for capillary lateral flow.

STEP 4

Read at 7 Mins

Read visual lines strictly at 7 minutes. Results become invalid after 10 minutes due to post-reaction chemical shifts.

Visual Results Determination Guide:

Negative (-): Control line (C), T1 line (Tetracyclines), and T2 line (β-Lactams) all appear dark and distinct. (Both classes below cutoff).
T1 Positive (+): T1 line is significantly lighter than C line or invisible → Tetracyclines residue detected.
T2 Positive (+): T2 line is significantly lighter than C line or invisible → β-Lactams residue detected.
T1 & T2 Positive (+): Both T1 and T2 lines are lighter than C line or invisible → Both target classes detected.
Invalid: Control (C) line fails to appear, regardless of T1 or T2 line presence. Re-testing with a new strip is required.

6. Performance & Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

Qualitative Screening Purpose: Engineered strictly for rapid qualitative prescreening and preliminary field audits. Confirmatory laboratory testing is recommended if necessary.
Air Exposure Limit: Use strips within 30 minutes after pulling from vial to prevent dampness and loss of reactivity.
Meticulous Pipetting: Thoroughly resuspend dry microwell reagents via repeated fluidic pipetting; improper mixing impairs line color development.
Environmental Limits: Maintain operating relative humidity at 30%–80%. Avoid direct wind currents and testing above 40°C.

7. Included Components & Auxiliary Equipment

Kit Components Included: 96 Test strips, 96 Precoated Microwells, and 1 User Manual.
Required Auxiliary Equipment (Not Provided): Micropipettes (150 µL), timer, and incubator (recommended if ambient temperature <20°C).

Request Samples & Ordering Information

Evaluate the FSTest® β-Lactams + Tetracyclines Rapid Test Strip in your dairy quality control workflows. Free evaluation sample packs are available for certified testing laboratories and dairy producers.

Email: service@fstestcorp.com | Website: www.fstestcorp.com

User manual

β-Lactams+Tetracyclines Rapid Test Strip User Manual.pdf Download