Pesticide Combo Card(Pesticide Test Card)
Brand: FSTest
10–15 mins Rapid Reaction Time | ChE Inhibition Cholinesterase Assay | Color Change Blue = Negative (Safe) | Instrument-Free On-Site Field Test |
The FSTest Pesticide Combo Card(Pesticide Test Card) is a fast, reliable, and instrument-free rapid test card designed for on-site screening of organophosphate and carbamate pesticide residues in fresh vegetables, fruits, tea, and agricultural produce.
Based on the standard Cholinesterase (ChE) enzyme inhibition method, organophosphate and carbamate pesticides inhibit cholinesterase activity, preventing color development. If the sample is clean, the enzyme reacts with the chromogenic substrate to turn sky blue (Negative). If toxic pesticide residues exceed safety limits, the card remains white or light yellow (Positive). The test provides results in 10 to 15 minutes, making it an ideal field screening solution for markets, farms, and food safety regulators.
Rapid Field Screening: Delivers definitive qualitative Pass/Fail results in 10–15 minutes directly at wholesale markets or farm gates.
No Instrument Required: Clear visual colorimetric reading requires zero expensive laboratory analyzers or power sources.
Dual-Group Pesticide Target: Simultaneously screens for two major toxic pesticide families—Organophosphates and Carbamates.
User-Friendly Operation: Minimal sample preparation—simply extract with water/buffer, apply drops to the reagent pad, fold the card, and observe color development.
Highly Cost-Effective: Reduces screening costs by over 90% compared to GC-MS or LC-MS laboratory chemical chromatography.
| Product Name | Pesticide Combo Card(Pesticide Test Card) |
|---|---|
| Test Principle | Enzyme Inhibition Rate Method (Cholinesterase + Chromogen) |
| Target Residues | Organophosphate and Carbamate Pesticides (e.g., Dichlorvos, Methamidophos, Carbofuran, Carbaryl, etc.) |
| Sensitivity / Detection Limit | 0.01 – 0.50 ppm / mg/kg (Complies with national maximum residue limits / MRLs) |
| Test Reaction Time | 10 – 15 minutes |
| Result Interpretation | Blue = Negative (Safe) | Light Blue / White = Positive (Pesticide Residue Present) |
| Shelf Life & Storage | 12 months; store refrigerated at 2–8°C in a dry, dark place |
Widely used across raw agricultural products, supply chain check-points, and retail markets:
STEP 1 Extract Cut 5 g sample, add 10 mL extraction buffer/water, shake for 1–2 min, and let stand for 3–5 min. | STEP 2 Inoculate & Pre-incubate Drip 1–2 drops of extract onto the red enzyme pad of the card; incubate at 37°C or body heat for 10 min. | STEP 3 Fold & React Pinch the card to fold white substrate pad onto red enzyme pad; hold for 3 minutes. | STEP 4 Read Color Sky Blue = Negative (Safe); Light Blue/White = Positive (Excess Pesticide Residue). |
The FSTest Pesticide Combo Card(Pesticide Test Card) delivers reliable enzyme inhibition accuracy for rapid screening:
Regulatory Method Compliance: Formulated in compliance with national rapid test standards for pesticide residue enzyme inhibition (e.g., GB/T 5009.199).
High Enzyme Stability: Highly active, stabilized cholinesterase reagent prevents ambient temperature degradation.
Low False Negative Rate: Calibrated to trigger positive color suppression at or near regulatory MRL toxicity levels.
Limit of Detection (LOD, ppm) for target organophosphate and carbamate pesticides across various vegetable matrices evaluated by Chiang Mai University:
| Sample | Carbofuran (LOD, ppm) | Methomyl (LOD, ppm) | Dichlorvos (LOD, ppm) | Carbaryl (LOD, ppm) |
|---|---|---|---|---|
| Yard long bean | 0.05 | 0.01 | 0.01 | 0.50 |
| Collard green | 0.05 | 0.01 | 0.01 | 0.50 |
| Bok choy | 0.05 | 0.01 | 0.01 | 0.50 |
| Napa cabbage | 0.05 | 0.05 | 0.01 | 0.50 |
| Cabbage | 0.05 | 0.05 | 0.01 | 0.50 |
Portable Incubator : Used for standardization of incubation in large-scale samples.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
Manufacturer: FSTest Co., Ltd.
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