5–8 mins Rapid Readout Time | 0.05–30 mg/kg Tunable Matrix LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–40°C) |
The FSTest Chlorothalonil Rapid Test Kit is a professional-grade rapid screening tool designed for the qualitative detection of chlorothalonil residues in fresh fruits and vegetables.
Chlorothalonil is a widely applied broad-spectrum fungicide. By bypassing the need for expensive, time-consuming laboratory infrastructure (such as HPLC or GC-MS), this field-ready kit simplifies complex agricultural sample extraction into a streamlined room-temperature workflow, delivering highly accurate visual result readouts within just 5 to 8 minutes for fresh produce supply chains and corporate quality control monitoring programs.
Ultra-Fast Testing Response: Complete the entire assay workflow and achieve highly accurate visual result readouts within 5 to 8 minutes post-preparation.
Highly Customizable Limits of Detection: Uniquely calibrated to adapt across different produce matrices, dynamically targeting a wide array of regulatory cut-off thresholds ranging from 0.05 mg/kg up to 30 mg/kg via standard or 2-step serial dilution protocols.
Fully Self-Contained Kit Architecture: Arrives fully packed with 10 test cassettes, 10 pre-loaded microwells, 10 centrifuge tubes (2 mL), 60 mL dedicated sample extraction solution, and 2 sampling bottles (30 mL).
High Specificity & Zero Cross-Reactivity: Rigorously validated to show zero cross-reactivity with other high-profile agricultural chemicals up to 10 mg/L (including carbendazim, imidacloprid, triazophos, and parathion).
Safe & Non-Toxic Formulation: Supplied with a certified safe and user-friendly extraction solution for hassle-free field testing.
| Product Name | Chlorothalonil Rapid Test Kit (Colloidal Gold) |
|---|---|
| Target Analyte Compound | Chlorothalonil (CAS: 1897-45-6) |
| Detection Principle | Competitive Inhibition Lateral Flow Immunochromatography |
| Pack Size | 10 tests / kit |
| Assay Run Time & Volume | 5–8 minutes (results invalid after 8 minutes); 100 µL final prepared solution |
| Storage & Shelf Life | 12 months sealed at 4–40°C in a cool, dry place (Do not freeze) |
| Applicable Fruit & Vegetable Commodity | Target LOD (mg/kg) | Supernatant X (µL) | Extracting Sol. Y (µL) |
|---|---|---|---|
| Fresh Lily Bulb | 0.05 mg/kg | 100 µL | 100 µL |
| Potato, Peach, Lychee, Banana | 0.2 mg/kg | 100 µL | 300 µL |
| Carrot, Celeriac, Ginger | 0.3 mg/kg | 100 µL | 300 µL |
| Cherry | 0.5 mg/kg | 50 µL | 350 µL |
| Mandarin, Tangerine, Orange, Apple, Pear | 1.0 mg/kg | 20 µL | 280 µL |
| Vegetable Soybean, Garlic Stalk, Radish | 2.0 mg/kg | 10 µL | 390 µL |
| Pickling Cucumber (Gherkin) | 3.0 mg/kg | 10 µL | 590 µL |
| Applicable High LOD Produce Commodity | Target LOD (mg/kg) | Mixture X (µL) | Extracting Sol. Y (µL) |
|---|---|---|---|
| Broccoli, Spinach, Pak Choi, Baby Bok Choy, Green Vegetable, Leaf Lettuce, Celery, Chinese Cabbage, Tomato, Eggplant, Pepper, Bell Pepper, Cucumber, Zucchini, Jointed Gourd, Bitter Melon, Loofah, Wax Gourd, Pumpkin, Squash, Cowpea, Lingonberry, Strawberry, Watermelon, Thin-skinned Melon, Reticulated Melon, Hami Melon, Bailan Melon, Muskmelon, Pepino | 5.0 mg/kg | 50 µL | 450 µL |
| Snow Pea, Cherry Tomato | 7.0 mg/kg | 20 µL | 280 µL |
| Onion, Green Onion, Cauliflower, Water Spinach, Grape | 10.0 mg/kg | 20 µL | 380 µL |
| Chinese Kale (Gai Lan) | 15.0 mg/kg | 10 µL | 290 µL |
| Gooseberry, Papaya, Currant | 20.0 mg/kg | 10 µL | 390 µL |
| Choy Sum | 30.0 mg/kg | 10 µL | 590 µL |
Extensively validated across over 50 fresh fruit, vegetable, and tuber commodities:
STEP 1 Comminution Cut fresh sample into 0.5 cm pieces. Weigh 2.0 ± 0.1 g into sampling bottle, add 3 mL extracting solution, shake 1 min, let stand 1 min. | STEP 2 Matrix Dilution Execute the specific Standard or Two-Step Serial Dilution protocol (mix 20 µL primary + 180 µL sol, then blend X µL + Y µL) based on produce type. | STEP 3 Microwell & Load Ensure 20–40°C environment. Pipette 100 µL final test solution into microwell, mix 3–5 times, stand 3 min. Transfer all to cassette sample well. | STEP 4 Read at 5–8 Mins Interpret visual lines strictly at 5–8 minutes. Visual readouts become strictly invalid after 8 minutes due to late chemical shifts. |
Negative (-): Both Control (C) line and Test (T) line develop strong, distinct red bands. Chlorothalonil residue is safely below the targeted matrix LOD limit.
Positive (+): Control (C) line develops normally, but Test (T) line is visibly lighter or fails to appear → Chlorothalonil residue ≥ targeted matrix LOD limit.
Invalid: Control (C) line fails to show any visible color band. Re-testing with a new cassette is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Dilution Protocol Accuracy: Ensure exact execution of Standard Protocol vs. Two-Step Serial Protocol based on matrix type to match target regulatory LOD cutoffs.
Time-Sensitive Exposure: Perform test within 1 hour after removing cassette from foil pouch. Read visual results strictly between 5–8 minutes.
Kit Components Included: 10 Test cassettes, 10 Pre-loaded microwells, 10 Centrifuge tubes (2 mL), Sample Extracting Solution (60 mL) x1, 30 mL Sampling bottles (x2), User Manual.
Required Equipment (Not Provided): Scissors, electronic balance (2.0 ± 0.1 g precision), micropipettes (X & Y µL, 100 µL), vortexer, centrifuge (optional, for turbid extracts), and timer.