The FSTest Acetamiprid Rapid Test Kit is an advanced, competitive lateral flow immunochromatographic assay engineered for rapid qualitative screening of acetamiprid pesticide residues across an extensive range of fruits and vegetables.

Acetamiprid is a widely applied neonicotinoid insecticide subject to rigorous Maximum Residue Limits (MRLs) globally. Featuring an ultra-sensitive baseline LOD of 0.02 mg/kg, intuitive multi-matrix dilution protocols aligned with commodity-specific MRLs, and visual readouts in just 5 to 8 minutes, this kit provides agricultural farms, wholesale produce markets, customs quarantine checkpoints, and food testing laboratories with an indispensable, highly mobile screening solution without relying on heavy LC-MS/MS instrumentation.

2. Key Benefits

Ultra-High Analytical Sensitivity (0.02 mg/kg Baseline LOD): Detects trace residues down to 0.02 mg/kg, ensuring compliance with strict regulatory surveillance standards across leafy, bulb, and fruiting commodities.
Fast 5–8 Minute Visual Turnaround: Delivers rapid on-site qualitative determinations within 5 to 8 minutes post-application, drastically accelerating intake inspection and harvest dispatch decisions.
Comprehensive Multi-Matrix Protocol: Features fully calibrated dilution ratios covering over 40+ agricultural produce matrices (bulb vegetables, solanaceous fruits, brassicas, legumes, leafy greens, stone fruits, tropical fruits, and berries).
High Analytical Selectivity: Zero cross-reactivity observed against 10 mg/L of common agricultural pesticides (Chlorpyrifos, Carbofuran, Cyromazine, Clothianidin, Dinotefuran, Thiabendazole, and Triazophos).
Eco-Friendly & Non-Toxic Formulation: Utilizes a safe, non-toxic aqueous extraction solution that protects operational personnel while simplifying routine chemical waste disposal.

3. Technical Specifications & LOD Spectrum

Product Name	Acetamiprid Rapid Test Kit
Catalog Number	23135
Target Analyte Compound	Acetamiprid (CAS: 135410-20-7; 160430-64-8 \| C₁₀H₁₁ClN₄ \| 222.67 g/mol)
Detection Principle	Competitive Lateral Flow Immunochromatography (Colloidal Gold)
Pack Size	10 tests / kit
Assay Run Time & Volume	5–8 minutes (results invalid after 8 minutes); 100 µL prepared sample solution
Storage & Shelf Life	12 months sealed at room temperature in a cool, dark, dry place (Do not freeze)

Commodity-Specific Matrix Dilution & Target LOD Alignment Guide

Applicable Commodity Classification & Sample Types	Targeted LOD	Supernatant (µL)	Extracting Solution (µL)
Bulb vegetables (excl. garlic, onion, Chinese chives, scallion, green garlic, garlic scapes, lily bulb)	0.02 mg/kg	100 µL	0 µL
Garlic, fresh lily bulb, fresh lotus seed, lotus root	0.05 mg/kg	100 µL	150 µL
Onion, broccoli	0.1 mg/kg	100 µL	400 µL
Solanaceous fruits (pepper), zucchini, chieh-qua, wax gourd, bayberry, watermelon, melon, pitaya	0.2 mg/kg	100 µL	900 µL
Pod-inedible legume vegetables (edamame, pea)	0.3 mg/kg	50 µL	700 µL
Headforming-Brassicas (excl. cauliflower & broccoli), pod-edible legumes (cowpea, sword bean)	0.4 mg/kg	50 µL	950 µL
Cabbage, cauliflower, bitter melon, kidney bean, broad bean, radish, citrus fruits (tangerine, mandarin, orange, lemon, kumquat), grape, papaya	0.5 mg/kg	20 µL	480 µL
Garlic scapes	0.7 mg/kg	20 µL	680 µL
Apple	0.8 mg/kg	20 µL	780 µL
Pak choi, Chinese cabbage, tomato, eggplant, sweet pepper, okra, cucumber, pumpkin, snap pea, stem lettuce (celtuce)	1.0 mg/kg	20 µL	980 µL
Leafy vegetables (sweet potato leaf, red amaranth, garland chrysanthemum, water spinach, mustard greens, baby bok choy)	1.5 mg/kg	20 µL	1480 µL
Two-Step Protocol: Chinese chives, green garlic, pome fruits (pear, loquat, hawthorn), stone fruits (peach, plum, cherry), berries (strawberry, blueberry, kiwi), tropical fruits (lychee, longan, mango, guava, carambola), melons	2.0 mg/kg	100 µL (pre-dilute)	100 µL
Two-Step Protocol: Flowering cabbage, celery, watercress, banana	3.0 mg/kg	100 µL (pre-dilute)	200 µL
Two-Step Protocol: Chinese kale, spinach, lettuce, stem lettuce leaves	5.0 mg/kg	100 µL (pre-dilute)	400 µL

Two-Step Pre-Dilution Procedure (for 2.0, 3.0, and 5.0 mg/kg LOD Targets):

Step A (Primary Pre-Dilution): Pipette 20 µL of sample supernatant, add 980 µL of Extracting Solution, and mix thoroughly.
Step B (Working Solution Dilution): Take 100 µL of the Step A pre-diluted mixture and add the corresponding Extracting Solution volume (100 µL for 2.0 mg/kg; 200 µL for 3.0 mg/kg; 400 µL for 5.0 mg/kg). Vortex for 10 seconds before testing.

4. Applicable Sample Types

Extensively validated across over 40+ fresh fruit and vegetable commodities:

Bulb & Allium Vegetables (Garlic, onion, Chinese chives, scallion, green garlic, garlic scapes, lily bulb) Brassica & Leafy Greens (Cabbage, cauliflower, broccoli, pak choi, Chinese cabbage, spinach, celery, kale, lettuce) Solanaceous, Cucurbits & Legumes (Tomatoes, eggplants, peppers, cucumber, zucchini, pumpkin, edamame, peas, cowpeas) Fruits & Melons (Apple, pear, peach, plum, strawberry, blueberry, citrus, watermelon, melon, dragon fruit, mango, banana)

5. Test Procedure

STEP 1

Extraction

Chop sample into 0.5 cm pieces. Weigh 1.0 g (±0.1 g) into a 30 mL sampling bottle, add 10 mL Extracting Solution, shake 1 min, and let stand 1 min. (Centrifuge if turbid).

STEP 2

Matrix Dilution

In a 2 mL tube, prepare test solution according to the commodity dilution table (single-step or two-step pre-dilution) and vortex 10 seconds to mix thoroughly.

STEP 3

Load Cassette

Ensure 20–40°C environment. Place cassette on a level surface and pipette exactly 100 µL of prepared test solution directly into sample well. Start timing.

STEP 4

Read at 5–8 Mins

Interpret visual lines strictly between 5 and 8 minutes. Visual readouts become completely invalid after 8 minutes due to late chemical shifts.

Visual Results Determination Guide:

Negative (-): Both Test (T) line and Control (C) line appear clearly. T line color intensity is equal to or darker than C line → Acetamiprid residue is absent or below the commodity's specific LOD threshold.
Positive (+): Control (C) line appears distinctly, while Test (T) line is invisible or visibly weaker/lighter than C line → Acetamiprid residue is present at or above the designated threshold limit.
Invalid: Control (C) line fails to appear, regardless of whether T line is visible. Re-test using a brand new cassette.

6. Performance & Crucial Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

Qualitative Screening Purpose: Engineered strictly for rapid qualitative screening; confirmatory quantitative testing should be performed via LC-MS/MS when required.
Cross-Reactivity Alert: Zero cross-reactivity observed against 10 mg/L of common pesticides (Chlorpyrifos, Carbofuran, Cyromazine, Clothianidin, Dinotefuran, Thiabendazole, Triazophos); samples containing 2 mg/L Imidacloprid show positive cross-reactivity due to shared neonicotinoid structures.
Moisture Protection: Begin testing within 1 hour after removing the test cassette from the protective foil pouch to prevent ambient humidity from degrading the strip membrane.
Strict Time Window: Read visual test results strictly between 5–8 minutes after sample application; results observed after 8 minutes are invalid.

7. Included Components & Auxiliary Equipment

Kit Components Included: 10 Test Cassettes (individually sealed with desiccant), 1 bottle of Acetamiprid Extracting Solution, 10 Centrifuge Tubes (2 mL), 2 Sampling Bottles (30 mL), User Manual.
Required Equipment (Not Provided): Scissors/knives, precision analytical balance (±0.1 g), micropipettes (20 µL, 100 µL, 1000 µL range) & tips, timer, vortex mixer, and centrifuge (optional, for turbid extracts).

Request Samples & Ordering Information

Evaluate the FSTest Acetamiprid Rapid Test Kit in your agricultural harvest receiving and food export compliance screening workflows. Free evaluation sample packs are available for certified testing laboratories, fresh produce exporters, and agricultural cooperatives.

Email: service@fstestcorp.com | Website: www.fstestcorp.com