The FSTest Imidacloprid Rapid Test Kit is a professional-grade rapid screening tool engineered for the qualitative detection of imidacloprid residues in fresh fruits, vegetables, and leafy greens.

Imidacloprid is a widely utilized neonicotinoid insecticide. By bypassing the need for complex, time-consuming laboratory infrastructure (such as HPLC or GC-MS), this field-ready kit compresses complex agricultural sample extraction into a simplified room-temperature workflow, delivering reliable visual results within 8 to 10 minutes for fresh produce supply chains and corporate food safety compliance monitoring programs.

2. Key Benefits

Ultra-Fast Field Turnaround: Complete the entire assay workflow and achieve highly reliable visual result readouts within 8 to 10 minutes post-sample-preparation.
Highly Customizable Limits of Detection: Uniquely calibrated to adapt across different produce matrices, targeting regulatory cut-off thresholds ranging from 0.1 mg/kg up to 5 mg/kg via standard or 2-step serial dilution protocols.
Fully Self-Contained Kit Architecture: Packed with all required consumables—including 10 test cassettes, 20 centrifuge tubes (2 mL), 100 mL extracting solution, and 2 sampling bottles (30 mL).
Definite Visual Interpretation: Utilizes a competitive lateral flow format where an increasing insecticide concentration causes the Test (T) line to fade or disappear, removing subjective reading errors.
Ambient Transport & Easy Storage: Sealed kits can be safely kept in a cool, dry location between 4–30°C for up to 12 months.

3. Technical Specifications & LOD Spectrum

Product Name	Imidacloprid Rapid Test Kit (Colloidal Gold)
Target Analyte Compound	Imidacloprid (CAS: 138261-41-3 / 105827-78-9)
Detection Principle	Competitive Inhibition Lateral Flow Immunochromatography
Pack Size	10 tests / kit
Assay Run Time & Volume	8–10 minutes (results invalid after 10 minutes); 100 µL final prepared solution
Storage & Shelf Life	12 months sealed at 4–30°C in a cool, dry place (Do not freeze)

1. Direct Extraction Dilution Protocol (Standard Protocol: 0.1 – 2.0 mg/kg LOD)

Applicable Fruit & Vegetable Commodity	Target LOD (mg/kg)	Supernatant X (µL)	Diluent Y (µL)
Garlic, onion, balsam pear, wax gourd, pumpkin, kidney bean, vegetable soybean, sweet potato, bamboo shoots	0.1 mg/kg	100 µL	300 µL
Sweet potato leaves, Chinese cabbage, pepper, asparagus, carrots	0.2 mg/kg	100 µL	700 µL
Garlic scape, Brussels sprouts, cabbage, chieh-qua, silk melon, broad bean, pea, rhizome vegetables (except carrot), potatoes, Zizania	0.5 mg/kg	950 µL	50 µL
Chinese chive, cabbage, cauliflower, broccoli, Chinese kale, lettuce, eggplant, pepper, cucumber, zucchini	1.0 mg/kg	780 µL	20 µL
Common cowpea	2.0 mg/kg	790 µL	10 µL

2. Two-Step Serial Dilution Protocol (High LOD Leafy Matrices: 5.0 mg/kg LOD)

Applicable High LOD Commodity	Target LOD (mg/kg)	Sequential Two-Step Dilution Protocol
Spinach, kales	5.0 mg/kg	Step 1: Pipette 100 µL primary supernatant + 900 µL extracting solution, swirl 30 s. Step 2: Pipette 50 µL solution from Step 1 + 950 µL extracting solution, swirl 30 s for final test.

4. Applicable Sample Types

Extensively validated across diverse agricultural produce matrices:

Bulb & Root Vegetables (Garlic, onion, carrot, potato, ginger, sweet potato) Brassica & Leafy Greens (Cabbage, broccoli, kale, spinach, chive, lettuce) Solanaceous, Cucurbit & Legumes (Pepper, eggplant, cucumber, pumpkin, peas, cowpea)

5. Test Procedure

STEP 1

Comminution

Cut fresh sample into 0.5 cm pieces. Weigh 1.0 ± 0.1 g into 30 mL sampling bottle, add 5 mL extracting solution, shake 1 min, let stand 1 min.

STEP 2

Matrix Dilution

Execute the specific Standard or Two-Step Serial Dilution protocol as defined in the tables above based on the produce type being tested.

STEP 3

Load Cassette

Ensure 20–60°C environment. Pipette 100 µL of final prepared matrix-diluted solution into round sample well on cassette. Start timing.

STEP 4

Read at 8–10 Mins

Interpret visual lines strictly at 8–10 minutes. Visual readouts become strictly invalid after 10 minutes due to late chemical shifts.

Visual Results Determination Guide:

Negative (-): Both Control (C) line and Test (T) line develop strong, distinct red bands. Imidacloprid residue is safely below the targeted matrix LOD limit.
Positive (+): Control (C) line develops normally, but Test (T) line is visibly lighter or fails to appear → Imidacloprid residue ≥ targeted matrix LOD limit.
Invalid: Control (C) line fails to show any visible color band. Re-testing with a new cassette is required.

6. Performance & Crucial Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Dilution Protocol Accuracy: Ensure exact execution of Standard Protocol vs. Two-Step Serial Protocol based on matrix type to match target regulatory LOD cutoffs.
Time-Sensitive Exposure: Perform test within 1 hour after removing cassette from pouch. Read visual results strictly between 8–10 minutes.

7. Included Components & Auxiliary Equipment

Kit Components Included: 10 Test cassettes, 2 mL Centrifuge tubes (x20), Extracting solution (100 mL) x1, 30 mL Sampling bottles (x2), User Manual.
Required Equipment (Not Provided): Scissors, electronic balance (1.0 ± 0.1 g precision), micropipettes (X & Y µL, 100 µL), vortexer, centrifuge (optional, for turbid extracts), and timer.

Request Samples & Ordering Information

Evaluate the FSTest Imidacloprid Rapid Test Kit (Colloidal Gold) in your fresh produce supply chain and export quality assurance workflows. Free evaluation sample packs are available for certified testing laboratories and agricultural exporters.

Email: service@fstestcorp.com | Website: www.fstestcorp.com