Thiamethoxam Rapid Test Kit
Brand: FSTest
CatNO: 23110
Specifications: 10 tests/kit.
Shelf life: 12 months.
Application: For the qualitative detection of thiamethoxam residues in fresh vegetables and fruits.
Thiamethoxam Rapid Test Kit
Thiamethoxam Rapid Test Kit


Thiamethoxam Rapid Test Kit

Competitive Lateral Flow Assay for Neonicotinoid Insecticides
PESTICIDE RESIDUE SCREENING
       Brand: FSTest
5–8 mins
Rapid Readout Time
0.01–10 mg/kg
Dynamic Multi-Tier LOD
10 Tests
Complete Kit Pack
12 Months
Shelf Life (4–30°C)
1. Product Overview

The FSTest Thiamethoxam Rapid Test Kit is a professional-grade, rapid screening tool engineered for the qualitative detection of thiamethoxam residues in fresh vegetables and fruits.

Thiamethoxam is a widely monitored second-generation neonicotinoid insecticide. By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural pesticide extraction into a simplified room-temperature workflow, delivering highly reliable visual results in just 5 to 8 minutes for produce logistics, harvest collection points, and corporate food safety compliance screening programs.

2. Key Benefits
  • High-Speed Field Turnaround: Complete the entire screening assay and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.

  • Dynamic Multi-Tier Detection Limits: Calibrated to precisely target regulatory cut-off thresholds ranging from an ultra-sensitive 0.01 mg/kg up to 10 mg/kg by utilizing a sophisticated multi-step supernatant dilution protocol.

  • Extensive Commodity Matrix Matching: Officially validated across 30+ produce commodities including legumes, young corn spears, bananas, brassica, melons, solanaceous, stone fruits, and leafy greens.

  • Comprehensive Kit Architecture: Fully equipped with 10 test cassettes, 20 centrifuge tubes (2 mL), 2 sampling bottles (30 mL), and an extra-large 100 mL bottle of dedicated extracting solution.

  • Reliable Ambient Preservation: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for up to 12 months.

3. Technical Specifications & LOD Spectrum
Product NameThiamethoxam Rapid Test Kit
Target Analyte CompoundThiamethoxam (CAS: 153719-23-4 | C₈H₁₀ClN₅O₃S | 291.71 g/mol)
Detection PrincipleCompetitive Inhibition Lateral Flow Immunochromatography
Pack Size10 tests / kit
Assay Run Time & Volume5–8 minutes (results invalid after 8 minutes); 100 µL final prepared solution
Storage & Shelf Life12 months sealed at 4–30°C in a cool, dry place (Do not freeze)
Advanced Commodity Sample Multi-Tier Dilution Protocol & LOD Spectrum
Applicable Agricultural Produce CommodityTargeted LODStep 1: Primary MixtureStep 2: Second Multiplier
Pod non-edible legume vegetables, young corn spears0.01 mg/kgDirect original supernatant/
Banana0.02 mg/kg100 µL Supernatant + 100 µL Extracting/
Chinese kale, okra, melon, etc.0.2 mg/kg980 µL Supernatant + 20 µL Extracting100 µL Step-1 + 300 µL Extracting
Cabbage, bitter melon, luffa, wax gourd, pumpkin, potato, etc.0.2 mg/kg950 µL Supernatant + 50 µL Extracting/
Onion, pod edible beans (excl. beans), Rhizome veggies (excl. turnip), apple, pear0.3 mg/kg870 µL Supernatant + 30 µL Extracting/
Solanaceous vegetables (except tomato, eggplant, pepper, okra)0.7 mg/kg20 µL Supernatant + 980 µL Extracting500 µL Step-1 + 200 µL Extracting
Flowering Chinese cabbage, tomato, pepper, chieh-qua, stem lettuce, celery, stone fruit1.0 mg/kg980 µL Supernatant + 20 µL Extracting100 µL Step-1 + 100 µL Extracting
Brassica vegetables (except cabbage, coconut veggies, Chinese kale, spinach)5.0 mg/kg980 µL Supernatant + 20 µL Extracting100 µL Step-1 + 900 µL Extracting
Phaseolus vulgaris7.0 mg/kg980 µL Supernatant + 20 µL Extracting50 µL Step-1 + 650 µL Extracting
Leeks, leaf lettuce10.0 mg/kg980 µL Supernatant + 20 µL Extracting50 µL Step-1 + 950 µL Extracting
4. Applicable Sample Types

Extensively validated across diverse agricultural produce categories:

Legumes & Corn (Edible/non-edible pod beans, Phaseolus vulgaris, young corn spears)    Brassica, Leafy & Stems (Cabbage, Chinese kale, flowering cabbage, spinach, leeks, lettuce, celery, stem lettuce)    Solanaceous, Cucurbits & Roots (Tomatoes, peppers, okra, bitter melon, luffa, wax gourd, pumpkin, potato, onion, ginger)    Fruits & Melons (Bananas, apples, pears, stone fruits, melons)
5. Test Procedure
STEP 1
Extraction
Cut fresh crop into 0.5 cm pieces. Weigh 1 g into sampling bottle, add 6 mL extracting solution, shake 1 min, let stand 1 min. (Centrifuge if cloudy).
STEP 2
Multi-Tier Dilution
Execute the exact Step 1 and Step 2 multi-tier matrix dilution protocol in 2 mL tubes as defined in the guide table for the commodity type.
STEP 3
Load Cassette
Ensure 20–40°C environment. Mount exactly 100 µL of final prepared sample solution directly into the sample hole of cassette. Start timing.
STEP 4
Read at 5–8 Mins
Interpret visual lines strictly at 5–8 minutes. Visual readouts become strictly invalid after 8 minutes due to late chemical shifts.
Visual Results Determination Guide:
  • Negative (-): Both Control (C) line and Test (T) line develop color normally. Thiamethoxam residue is safely below the targeted matrix LOD limit.

  • Positive (+): Control (C) line develops normally, but Test (T) line is visibly lighter or fails to appear → Thiamethoxam residue ≥ targeted matrix LOD limit.

  • Invalid: Control (C) line fails to show any visible color band. Re-testing with a new cassette is required.

6. Performance & Crucial Operational Controls

Adhere to strict operational parameters to ensure maximum screening accuracy:

  • Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.

  • Multi-Tier Dilution Accuracy: Strictly perform Step 1 and Step 2 dilution ratios corresponding to the specific produce commodity to calibrate reading against exact regulatory MRL cutoffs.

  • Time-Sensitive Exposure: Perform test within 1 hour after removing cassette from foil pouch. Read visual results strictly between 5–8 minutes.

7. Included Components & Auxiliary Equipment
  • Kit Components Included: 10 Test cassettes, 20 Centrifuge tubes (2 mL), Extracting solution (100 mL) x1, 30 mL Sampling bottles (x2), User Manual.

  • Required Equipment (Not Provided): Scissors, electronic balance (1 g precision), micropipettes (X & Y µL, 100 µL), vortexer, centrifuge (optional, for turbid extracts), and timer.

Request Samples & Ordering Information
Evaluate the FSTest Thiamethoxam Rapid Test Kit in your fresh produce supply chain and export quality assurance workflows. Free evaluation sample packs are available for certified testing laboratories and agricultural exporters.

     Email: service@fstestcorp.com | Website: www.fstestcorp.com

Certification & Validation
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