5–8 mins Rapid Readout Time | 0.01–10 mg/kg Dynamic Multi-Tier LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Thiamethoxam Rapid Test Kit is a professional-grade, rapid screening tool engineered for the qualitative detection of thiamethoxam residues in fresh vegetables and fruits.
Thiamethoxam is a widely monitored second-generation neonicotinoid insecticide. By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural pesticide extraction into a simplified room-temperature workflow, delivering highly reliable visual results in just 5 to 8 minutes for produce logistics, harvest collection points, and corporate food safety compliance screening programs.
High-Speed Field Turnaround: Complete the entire screening assay and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.
Dynamic Multi-Tier Detection Limits: Calibrated to precisely target regulatory cut-off thresholds ranging from an ultra-sensitive 0.01 mg/kg up to 10 mg/kg by utilizing a sophisticated multi-step supernatant dilution protocol.
Extensive Commodity Matrix Matching: Officially validated across 30+ produce commodities including legumes, young corn spears, bananas, brassica, melons, solanaceous, stone fruits, and leafy greens.
Comprehensive Kit Architecture: Fully equipped with 10 test cassettes, 20 centrifuge tubes (2 mL), 2 sampling bottles (30 mL), and an extra-large 100 mL bottle of dedicated extracting solution.
Reliable Ambient Preservation: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for up to 12 months.
| Product Name | Thiamethoxam Rapid Test Kit |
|---|---|
| Target Analyte Compound | Thiamethoxam (CAS: 153719-23-4 | C₈H₁₀ClN₅O₃S | 291.71 g/mol) |
| Detection Principle | Competitive Inhibition Lateral Flow Immunochromatography |
| Pack Size | 10 tests / kit |
| Assay Run Time & Volume | 5–8 minutes (results invalid after 8 minutes); 100 µL final prepared solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Applicable Agricultural Produce Commodity | Targeted LOD | Step 1: Primary Mixture | Step 2: Second Multiplier |
|---|---|---|---|
| Pod non-edible legume vegetables, young corn spears | 0.01 mg/kg | Direct original supernatant | / |
| Banana | 0.02 mg/kg | 100 µL Supernatant + 100 µL Extracting | / |
| Chinese kale, okra, melon, etc. | 0.2 mg/kg | 980 µL Supernatant + 20 µL Extracting | 100 µL Step-1 + 300 µL Extracting |
| Cabbage, bitter melon, luffa, wax gourd, pumpkin, potato, etc. | 0.2 mg/kg | 950 µL Supernatant + 50 µL Extracting | / |
| Onion, pod edible beans (excl. beans), Rhizome veggies (excl. turnip), apple, pear | 0.3 mg/kg | 870 µL Supernatant + 30 µL Extracting | / |
| Solanaceous vegetables (except tomato, eggplant, pepper, okra) | 0.7 mg/kg | 20 µL Supernatant + 980 µL Extracting | 500 µL Step-1 + 200 µL Extracting |
| Flowering Chinese cabbage, tomato, pepper, chieh-qua, stem lettuce, celery, stone fruit | 1.0 mg/kg | 980 µL Supernatant + 20 µL Extracting | 100 µL Step-1 + 100 µL Extracting |
| Brassica vegetables (except cabbage, coconut veggies, Chinese kale, spinach) | 5.0 mg/kg | 980 µL Supernatant + 20 µL Extracting | 100 µL Step-1 + 900 µL Extracting |
| Phaseolus vulgaris | 7.0 mg/kg | 980 µL Supernatant + 20 µL Extracting | 50 µL Step-1 + 650 µL Extracting |
| Leeks, leaf lettuce | 10.0 mg/kg | 980 µL Supernatant + 20 µL Extracting | 50 µL Step-1 + 950 µL Extracting |
Extensively validated across diverse agricultural produce categories:
STEP 1 Extraction Cut fresh crop into 0.5 cm pieces. Weigh 1 g into sampling bottle, add 6 mL extracting solution, shake 1 min, let stand 1 min. (Centrifuge if cloudy). | STEP 2 Multi-Tier Dilution Execute the exact Step 1 and Step 2 multi-tier matrix dilution protocol in 2 mL tubes as defined in the guide table for the commodity type. | STEP 3 Load Cassette Ensure 20–40°C environment. Mount exactly 100 µL of final prepared sample solution directly into the sample hole of cassette. Start timing. | STEP 4 Read at 5–8 Mins Interpret visual lines strictly at 5–8 minutes. Visual readouts become strictly invalid after 8 minutes due to late chemical shifts. |
Negative (-): Both Control (C) line and Test (T) line develop color normally. Thiamethoxam residue is safely below the targeted matrix LOD limit.
Positive (+): Control (C) line develops normally, but Test (T) line is visibly lighter or fails to appear → Thiamethoxam residue ≥ targeted matrix LOD limit.
Invalid: Control (C) line fails to show any visible color band. Re-testing with a new cassette is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Multi-Tier Dilution Accuracy: Strictly perform Step 1 and Step 2 dilution ratios corresponding to the specific produce commodity to calibrate reading against exact regulatory MRL cutoffs.
Time-Sensitive Exposure: Perform test within 1 hour after removing cassette from foil pouch. Read visual results strictly between 5–8 minutes.
Kit Components Included: 10 Test cassettes, 20 Centrifuge tubes (2 mL), Extracting solution (100 mL) x1, 30 mL Sampling bottles (x2), User Manual.
Required Equipment (Not Provided): Scissors, electronic balance (1 g precision), micropipettes (X & Y µL, 100 µL), vortexer, centrifuge (optional, for turbid extracts), and timer.