E. coli STEC Count Plate
Catalog No.: 21208 | Brand: FSTest
15–24 hrs Fast Incubation Time | 1 CFU/g (mL) Pathogen Surveillance LOD | 24 Tests Self-Seal Foil Pack | 18 Months Shelf Life (4–10°C) |
The FSTest E. coli STEC Count Plate is a single-use, rapid microbial testing product developed using proprietary dry-film culture plate technology for the rapid screening and enumeration of Shiga toxin-producing Escherichia coli (STEC).
Utilizing imported, highly selective culture media as its core substrate alongside specific chromogenic enzymatic indicators, it confirms STEC contamination in a single-step incubation period of only 15 to 24 hours. By eliminating traditional culture media preparation, autoclave sterilization, and petri dish pouring, this count plate simplifies laboratory testing workflows for food inspection authorities, slaughterhouses, and food manufacturing enterprises.
High Sensitivity (1 CFU/g or mL LOD): Detects target microorganisms down to 1 CFU/g (mL) of sample homogenate, fully meeting international standards for foodborne pathogen surveillance.
Fast 15–24 Hour Results: Delivers definitive qualitative screening and colony counting in 15–24 hours, cutting turnaround time in half compared to conventional agar plating methods.
Intuitive Chromogenic Differentiation: Employs enzyme-specific visual color discrimination: typical E. coli STEC colonies develop a clear purple-red color, whereas non-STEC coliforms develop distinct blue colonies.
Ready-to-Use Self-Gelling Media: Requires no boiling, autoclaving, or agar cooling. Directly pipette 1.0 mL of sample homogenate onto the dry film for rapid gel rehydration.
Compact & Space-Efficient: Ultra-thin film format reduces incubator footprint and storage space by over 80% while minimizing biohazard autoclave waste volume.
| Product Name | E. coli STEC Count Plate |
|---|---|
| Catalog Number | 21208 / C898 |
| Target Microorganism | Shiga toxin-producing Escherichia coli (STEC / VTEC / EHEC) |
| Methodology | Selective Chromogenic Enzyme Dry-Film Count Plate |
| Inoculation Volume & Temp | 1.0 mL per plate (tested in duplicate); Incubate at 36 ± 1°C |
| Incubation Time | 15 – 24 hours |
| Pack Size & Storage | 24 tests / pack; 4–10°C refrigerated (room temperature transit ≤ 15 days permitted) |
| Shelf Life & Biosafety | 18 months sealed (use within 1 month after opening); BSL-2 facility recommended |
| Colony Appearance | Color & Morphological Feature | Diagnostic Identification |
|---|---|---|
| Target Colony Growth | Typical purple-red colonies | Positive for E. coli STEC |
| Non-Target Growth | Distinct blue colonies | Other Coliforms / Non-STEC |
| No Colony Growth | No colored colonies observed | Negative (< 1 CFU/g or mL) |
Optimized specifically for identifying and enumerating Shiga toxin-producing E. coli across key food sectors:
STEP 1 Homogenization Aseptically weigh 25 g (or 25 mL) sample into a sterile bag. Add 225 mL sterile saline or PBS (1:10 dilution) and homogenize 1–2 minutes using a stomacher. | STEP 2 Plate Inoculation Place plate flat on bench. Lift transparent top film and dispense exactly 1.0 mL homogenate onto center. Lower film carefully to avoid introducing bubbles. | STEP 3 Gelling & Spreading Allow plate to stand undisturbed for 5 minutes for media solidification, then press down gently with clean spreader. Inoculate duplicate plates per sample. | STEP 4 Incubate (15–24 hrs) Stack ≤ 12 plates in original self-seal bag, seal securely, and place horizontally (film side up) in incubator at 36 ± 1°C for 15–24 hours. Count purple-red colonies. |
Target Growth (Purple-Red Colonies): Presumptive positive for Shiga toxin-producing Escherichia coli (STEC). Confirmatory testing (e.g., PCR for stx1/stx2 genes) is recommended.
Non-Target Growth (Blue Colonies): Represents other coliforms or non-STEC bacteria. These colonies should not be counted as STEC.
No Colony Growth: Indicates target STEC count is below 1 CFU/g (or 1 CFU/mL). Sample passes foodborne pathogen screening.
Adhere to strict microbiological safety parameters to ensure reliable diagnostics:
BSL-2 Laboratory Facility: All testing and microbial manipulation procedures must be performed within a certified Biosafety Level 2 (BSL-2) laboratory due to the severe pathogenicity of STEC strains.
Biohazard Waste Disposal: Autoclave spent plates and biohazard waste at 121°C for at least 30 minutes or dispose of via certified biohazardous waste incineration.
Stacking & Condensation Control: Do not stack more than 12 plates in the incubator. Return plates into the original self-seal bag to prevent media dehydration during incubation.
Package Handling: Bring refrigerated pouches to room temperature before opening to avoid condensation. Reseal unused plates and use within 1 month.
Package Components Included: 24 E. coli STEC Count Plates (packaged in a resealable foil bag), User Instruction Manual.
Required Equipment (Not Provided): Stomacher/homogenizer & sterile bags, incubator (36 ± 1°C), sterile saline or PBS diluent, micropipette (1000 µL) & sterile tips, analytical balance, and autoclave.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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