Water Quality Coliform / Fecal Coliform Test Paper
Catalog No.: 21206/21207| Brand: FSTest
16–18 hrs Fast Incubation Readout | 20 MPN/L Strict Base Sensitivity LOD | Type I & II 10 mL & 1 mL Dual Formats | 12 Months Shelf Life (4–10°C) |
The FSTest Water Quality Coliform / Fecal Coliform Test Paper (Type I and Type II) is a standardized, rapid paper-sheet culture testing system engineered for the qualitative screening and statistical (MPN) enumeration of Total Coliforms and Fecal (Thermotolerant) Coliforms across diverse environmental and municipal water sources.
Formulated with targeted microbial nutrients, specific selective inhibitors against non-target background flora, sensitive pH indicators, and chromogenic enzymatic substrates, the test paper delivers definitive colorimetric results within 16 to 18 hours (or 18–24 hours). Demonstrating high concordance with traditional multi-tube fermentation (MTF) and membrane filtration methods, it eliminates bulky agar preparation and autoclave sterilization, providing environmental stations, waterworks, WWTPs, and disease control centers with an exceptionally mobile and cost-efficient monitoring platform.
High Regulatory Sensitivity (20 MPN/L LOD): Detects sanitary indicator coliforms down to 20 MPN/L, fully satisfying drinking water, surface water, and wastewater discharge monitoring standards.
Fast 16–18 Hour Turnaround: Yields conclusive colorimetric results within 16–18 hours, shortening the inspection window compared to standard 48–72 hour traditional broth procedures.
Flexible Dual-Format Architecture: Combines Type I (10 mL inoculation capacity for clean/source waters) and Type II (1 mL capacity for contaminated effluents), accommodating sample contamination spanning 7 orders of magnitude.
Dual-Target Temperature Selectivity: Easily differentiates Total Coliforms (incubated at 37 ± 1°C) from Fecal Coliforms (incubated at 44.5 ± 0.5°C) using the exact same test paper substrate.
Built-in Interference Elimination: Fully compatible with chemical pre-treatments using sodium thiosulfate (neutralizing residual chlorine) and EDTA-Na2 (chelating toxic heavy metals) to prevent false-negative inhibition.
Comprehensive MPN Statistical Framework: Accompanied by standard 15-tube/paper MPN index tables (5×10mL, 5×1mL, 5×0.1mL scheme) and scalable dilution calculation formula C = (100 × M) / Q.
| Product Name | Water Quality Coliform / Fecal Coliform Test Paper |
|---|---|
| Catalog Numbers | 21206 (Type I: 10 mL capacity) / 21207 (Type II: 1 mL capacity) |
| Target Microorganisms | Total Coliforms (37°C) and Fecal / Thermotolerant Coliforms (44.5°C) |
| Methodology | Enzyme Substrate Chromogenic / Selective Inhibition Paper Sheet |
| Base Limit of Detection (LOD) | 20 MPN/L |
| Incubation Parameters | Total Coliforms: 37 ± 1°C for 18–24 h | Fecal Coliforms: 44.5 ± 0.5°C for 18–24 h (immediate placement) |
| Assay Scheme & Sample pH | Standard 15 test papers per sample (5 papers × 3 dilution tiers); pH 7.0–8.0 |
| Storage & Shelf Life | 12 months sealed at 4–10°C (transit ≤ 15 days at ambient temp permitted); 1 month after opening |
| Water Sample Classification | Recommended Inoculation Volumes (mL) | Recommended Paper Combination |
|---|---|---|
| Lake Water / Reservoir Source Water | 10 mL, 1 mL, 0.1 mL (Total: 55.5 mL) | Type I (5 pcs) + Type II (10 pcs) |
| River Water | 1 mL, 0.1 mL (10⁻¹), 0.01 mL (10⁻²) | Type II (15 pcs) |
| Domestic Wastewater (Untreated raw sewage) | 0.01 mL (10⁻²), 0.001 mL (10⁻³), 0.0001 mL (10⁻⁴) | Type II (15 pcs) |
| Treated Hospital / Medical Wastewater | 1 mL, 0.1 mL (10⁻¹), 0.01 mL (10⁻²) | Type II (15 pcs) |
| Livestock Breeding Wastewater (Raw manure sewage) | 0.0001 mL (10⁻⁴), 10⁻⁵ mL, 10⁻⁶ mL | Type II (15 pcs) |
Engineered for microbiological surveillance across the entire aquatic cycle:
STEP 1 Sampling & Prep Collect ~200 mL aseptically. Add sodium thiosulfate for chlorinated water or EDTA-Na2 for heavy metals. Adjust sample to pH 7.0–8.0 if outside range. | STEP 2 Inoculation Set up 15 papers across 3 decimal dilution tiers (5 papers/tier). Inoculate sample evenly until paper sheet is saturated, then gently smooth flat and label. | STEP 3 Incubation Total Coliform: 37 ± 1°C for 18–24 h (preliminary reading at 16–18 h). Fecal Coliform: immediately place in 44.5 ± 0.5°C incubator for 18–24 h without delay. | STEP 4 Read & Calculate Count positive sheets across 3 tiers (e.g., 5-3-1), retrieve base MPN index (M) from table, and calculate final concentration C = (100 × M) / Q. |
Positive (+): Red spots or halos appear and the surrounding area turns yellow; OR the entire paper turns uniform yellow (with or without red spots); OR paper partially turns yellow accompanied by red spots/halos → Active coliform fermentation confirmed.
Negative (-): Red spots appear on original purple background but with NO yellowing; OR no color change (retains original purple background); OR paper partially turns yellow without any red spots/halos → Negative for target coliforms.
Immediate Acid Interference Alert: If test paper turns yellow immediately upon sample application prior to incubation, acidic or oxidizing substances are present in the sample; adjust pH and re-test.
Adhere to strict microbiological surveillance protocols to ensure accurate MPN statistics:
Immediate 44.5°C Placement: For Fecal (Thermotolerant) Coliform testing, inoculated test papers must be placed into the 44.5 ± 0.5°C incubator immediately. Room-temperature lag leads to false-positive growth of environmental non-fecal coliforms.
Batch Negative Blank Run: Include a negative control for each testing batch using 15 test papers inoculated with sterile deionized water; all 15 sheets must remain completely negative.
Sampling Transit Time: Inoculate samples within 2 hours of sampling, or store at 0–4°C and process within 6 hours to preserve microbial viability and population ratios.
Biohazard Waste Decontamination: Autoclave spent test papers, pipette tips, and sample residues at 121°C for 20 minutes before disposal.
Product Packaging Formats: Type I Test Papers (10 mL capacity) and Type II Test Papers (1 mL capacity), packaged in sealed moisture-proof foil pouches with User Manual and 15-Tube MPN Index Table.
Required Equipment (Not Provided): Constant temperature incubators (37 ± 1°C and 44.5 ± 0.5°C), sterile sampling bottles, micropipettes (1 mL, 10 mL) & sterile tips, sterile dilution water/saline, sodium thiosulfate (for chlorine), and EDTA-Na2 (for heavy metals).
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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