5–8 mins Rapid Readout Time | 0.02 mg/kg Ultra-High LOD (Snow Pea) | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Carbendazim Rapid Test Kit is a lateral flow immunochromatographic assay designed for the rapid qualitative screening of carbendazim fungicide residues in various fruits and vegetables.
Carbendazim is a broad-spectrum benzimidazole fungicide widely used to control plant diseases; however, stringent Maximum Residue Limits (MRLs) are enforced globally due to potential toxicity risks. Utilizing pre-coated recombinant antigens on a test strip, this kit provides convenient, visual, and reliable screening results directly observed by the naked eye within 5 to 8 minutes without requiring complex laboratory instrumentation.
Ultra-High Sensitivity: Achieves an exceptional Limit of Detection (LOD) as low as 0.02 mg/kg in sensitive matrices such as snow peas.
Extensive Produce Compatibility: Optimized extraction and dilution protocols covering over 40 types of fruits and vegetables (berries, citrus, stone fruits, leafy greens, root veggies).
Rapid On-Site Screening: Streamlined extraction workflow delivering clear visual results within 5 to 8 minutes post-preparation.
High Specificity & Low Interference: Tested negative against 10 mg/L of chlorpyrifos, carbofuran, imidacloprid, bromophos, abamectin, or tebuconazole.
Convenient Room Temp Storage: Sealed kits can be safely stored in a cool, dry location between 4–30°C for up to 12 months.
| Product Name | Carbendazim Rapid Test Kit (Colloidal Gold) |
|---|---|
| Target Analyte Compound | Carbendazim (CAS: 10605-21-7 | C₉H₉N₃O₂ | 191.19 g/mol) |
| Detection Principle | Sandwich Lateral Flow Immunochromatography (Colloidal Gold) |
| Pack Size | 10 tests / kit |
| Assay Reaction Time & Volume | 5–8 minutes (results invalid after 8 minutes); 100 µL test solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Tested Produce Sample Matrix Category | Limit of Detection (LOD) |
|---|---|
| Snow peas | 0.02 mg/kg |
| Pickling cucumbers | 0.05 mg/kg |
| Carrots, Lotus root, Vegetable soybeans | 0.2 mg/kg |
| Bitter gourd | 0.3 mg/kg |
| Brussels sprouts, zucchini, green beans, asparagus, root mustard, lemon, grapefruit, fresh jujube, plum, cherry, blackberry, currant, strawberry, olive, fig, lychee, pineapple | 0.5 mg/kg |
| Leaf mustard, berries, small fruits (tree tomato, blueberry, mulberry, currant) | 1 mg/kg |
| Chinese chives, chili peppers, cucumber, peach, nectarine, apricot, mango, banana, watermelon | 2 mg/kg |
| Tomato, eggplant, pear, hawthorn, loquat, quince, grape | 3 mg/kg |
| Head lettuce, tangerine, mandarin, orange, apple, kiwi | 5 mg/kg |
| Longan | 10 mg/kg |
Extensively validated across diverse fruit and vegetable produce categories:
STEP 1 Extraction Cut sample into 0.5 cm pieces. Weigh 2 g into sampling bottle, add 6 mL Extracting Solution, shake 1 min, let stand 1 min. (Centrifuge if cloudy). | STEP 2 Matrix Dilution Pipette X µL supernatant and mix with Y µL Extracting Solution in a 2 mL tube according to the produce matrix dilution table. | STEP 3 Microwell & Load Ensure 20–40°C environment. Pipette 100 µL diluted solution into microwell, mix 3–5 times, stand 3 min. Transfer all to cassette sample well. | STEP 4 Read at 5–8 Mins Interpret visual lines strictly at 5–8 minutes. Visual readouts become strictly invalid after 8 minutes due to post-reaction shifts. |
Negative (-): Both Control (C) line and Test (T) line appear clearly. Carbendazim residue is safely below the matrix LOD threshold.
Positive (+): Control (C) line appears; Test (T) line does not appear or is noticeably lighter → Carbendazim residue ≥ matrix LOD threshold.
Invalid: Control (C) line fails to appear, regardless of T line presence. Re-testing with a new cassette is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Cross-Reactivity Profile: Negative against 10 mg/L chlorpyrifos, carbofuran, imidacloprid, bromophos, abamectin, tebuconazole; Positive for 0.04 mg/L benomyl, 0.25 mg/L thiophanate-methyl, 1 mg/L carbendazim, 2 mg/L methiram.
Strict Time Window: Read visual test results strictly between 5–8 minutes after loading the cassette; results observed after 8 minutes are invalid.
Kit Components Included: 10 Test cassettes, 10 Microwells, 10 Centrifuge tubes (2 mL), Extracting Solution (100 mL) x1, 30 mL Sampling bottles (x2), User Manual.
Required Equipment (Not Provided): Knives or scissors, micropipettes, electronic balance, timer, vortexer, centrifuge (optional, for turbid samples).