E.coli O157 Rapid Test Kit
Brand: FSTest
10–15 mins Strip Reaction Time | 1–10 CFU High Sensitivity (g/mL) | High Specificity Monoclonal Antibody | Clear Visual Two-Line Interpretation |
The FSTest E.coli O157 Rapid Test Kit is a highly sensitive immunochromatographic strip assay designed for rapid qualitative screening of Escherichia coli O157 (including E. coli O157:H7) in beef, raw meat, dairy, produce, and environmental samples.
Utilizing colloidal gold lateral flow immunoassay technology with highly specific anti-O157 monoclonal antibodies, this test strip delivers clear, visual results in just 10 to 15 minutes post-enrichment. It serves as an essential rapid prescreening tool for meat processors, fresh produce suppliers, and food safety testing laboratories to intercept STEC risk early and accelerate product release.
Rapid & Actionable Screening: Generates clear qualitative results in 10–15 minutes after sample enrichment, significantly cutting product quarantine times.
High Monoclonal Specificity: Specific monoclonal antibodies selectively target the O157 lipopolysaccharide antigen, minimizing cross-reactivity with non-O157 E. coli strains and background flora.
Intuitive Visual Readout: Simple two-line result format (Control line and Test line) enables straightforward manual interpretation without complex instrumentation.
User-Friendly Format: Individually foil-sealed cassettes/strips require minimal hands-on preparation time and zero specialized equipment.
Cost-Efficient Prescreening: Offers an economical screening alternative to PCR or lengthy multi-step agar isolation methods.
| Product Name | E.coli O157 Rapid Test Kit |
|---|---|
| Detection Target | Escherichia coli O157 (including E. coli O157:H7) |
| Test Principle | Lateral Flow Immunochromatography |
| Limit of Detection (LOD) | 1–10 CFU/g or mL (Enriched sample broth) |
| Strip Reaction Time | 10 – 15 minutes at room temperature |
| Result Interpretation | Negative: C Line only | Presumptive Positive: Both C and T Lines visible |
| Shelf Life & Storage | 18 months; store at 2–25°C in a dry place (Do not freeze) |
Critical for screening high-risk food industries and environmental hygiene programs:
STEP 1 Enrich Homogenize 25 g sample with 225 mL selective E. coli enrichment broth (mTSB + Novobiocin) and incubate per standard protocol. | STEP 2 Apply Pipette 2–3 drops (approx. 100 µL) of enriched sample broth into the sample well (S) of the test cassette. | STEP 3 React Allow the test strip to incubate at room temperature for 10 to 15 minutes for lateral flow color development. | STEP 4 Read Observe lines: C Line only = Negative; C & T Lines visible = Presumptive Positive for E. coli O157. |
The FSTest E.coli O157 Rapid Test Kit is validated to ensure high accuracy for pathogen screening:
High Method Agreement: Correlates strongly (> 95%) with standard reference culture methods (ISO 16654).
Target Specificity: Monoclonal antibody formulation ensures excellent differentiation against non-O157 E. coli strains and background microflora.
Strict Manufacturing Standards: Produced under ISO 9001, ISO14001 and ISO 45001 certified quality management systems.
Microbial Test Sampling Box: Portable kit containing pipettes, tubes, and sampling tools for environmental pathogen screening.
Email: service@fstestcorp.com | Website: www.fstestcorp.com
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