FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit – High Sensitivity, Accurate Quantification, and Rapid Analysis

The FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit is a highly sensitive enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of nitrofurazone metabolites (SEM) in animal tissues and seafood.  This test kit offers precise, low-detection limit results, making it an essential tool for food safety testing laboratories, regulatory agencies, and quality control departments.

Key Features & Benefits:

✔ High Sensitivity – Ultra-low detection limit of 0.04ppb, ensuring trace-level residue detection.

✔ Accurate Quantification – Provides precise concentration measurements for regulatory compliance.

✔ Simultaneous Batch Testing – 96-well format allows for high-throughput analysis, improving efficiency.

✔ Reliable & Reproducible – Average coefficient of variation ≤ 10%, ensuring test consistency.

✔ Easy Sample Preparation – Compatible with animal tissue and seafood samples with a simple extraction protocol.

✔ Complies with Global Standards – Ideal for food safety screening in compliance with EU, US, and international regulations.

Applications:

Designed for fast and precise detection of SEM residues in meat, poultry, fish, shrimp, and seafood products, ensuring food safety and compliance with international regulatory limits.

Testing Principle:

This ELISA test uses a competitive immunoassay approach, where SEM in the sample competes with enzyme-conjugated SEM for binding to specific antibodies.  The color intensity measured at 450nm is inversely proportional to the SEM concentration in the sample.  The results are then compared against a standard curve to determine SEM levels accurately.

How to Use:

1️⃣ Sample Preparation – Homogenize and extract the sample following the protocol.

2️⃣ Test Procedure:

Add 50μL of standard or sample to each test well.

Add 50μL of enzyme conjugate solution and 50μL of antibody solution.

Incubate at 20-28°C for 30 minutes in the dark.

Wash the wells 4-5 times and remove excess liquid.

Add 100μL of substrate solution, incubate for 30 minutes, then add 100μL of stop solution.

Measure absorbance at 450nm using a microplate reader.

3️⃣ Result Interpretation – Compare absorbance values with the standard curve to determine SEM concentration.

Why Choose FSTest SEM ELISA Kit?

✅ Enhanced Detection Accuracy – Detects SEM residues down to 0.04ppb, meeting stringent food safety standards.

✅ Optimized for Food Industry & Regulatory Testing – Ideal for food manufacturers, testing labs, and government agencies.

✅ Efficient Sample Analysis – Capable of testing multiple samples in a single run, reducing processing time.

Storage & Stability:

Store at 2-8°C in a sealed container.  Shelf life: 12 months.  Do not freeze.

Ensure food safety compliance with the FSTest Nitrofurazone Metabolite (SEM) ELISA Test Kit – the gold standard for highly sensitive, accurate, and cost-effective food residue screening.