FSTest Tetracyclines ELISA Test Kit – High-Sensitivity Detection for Banned Antibiotic Residues

Introduction:

The FSTest Tetracyclines ELISA Test Kit is a precise immunoassay designed for quantitative detection of tetracycline-class antibiotics (including tetracycline, chlortetracycline, and oxytetracycline) in animal tissues, seafood, honey, and milk. With a 2ppb detection limit across all matrices, this kit ensures compliance with global regulations prohibiting tetracycline residues in food products.

Key Features & Benefits:

✔ Comprehensive Detection - Covers 4 key tetracyclines including: Tetracycline (100% cross-reactivity), Oxytetracycline (80%), Chlortetracycline (45%), Doxycycline (10%)

✔ Ultra-Sensitive - 2ppb detection limit meets strictest global MRLs

✔ Multi-Matrix Validation - Certified for meat, seafood, dairy, and honey

✔ Regulatory Ready - Complies with EU/China/USDA zero-tolerance policies

✔ Efficient Workflow - 96-well format processes 20+ samples per run

✔ Simplified Protocols - Matrix-specific sample preparation methods

Applications:

Livestock and poultry antibiotic monitoring

Seafood and aquaculture safety testing

Dairy product quality control

Honey export compliance verification

Veterinary drug residue surveillance

Testing Principle:

Competitive ELISA measuring absorbance at 450nm (inversely proportional to tetracycline concentration) against a 5-point standard curve (0-2.7ppb).

How to Use:

1️⃣ Sample Preparation:

Meat/Seafood: HCl extraction + pH adjustment

Honey: Simple water dilution

Milk: Trichloroacetic acid precipitation

2️⃣ Test Procedure:

Add 50μL sample/standard

Add 50μL enzyme conjugate & antibody

Incubate 30min (20-28°C)

Wash 4-5 times

Add substrate, incubate 30min

Add stop solution

Read at 450nm

3️⃣ Result Interpretation:

Compare to standard curve (0.1-2.7ppb)

Apply 10× dilution factor for all matrices

Why Choose FSTest Tetracyclines ELISA Kit?

✅ Most Regulated Antibiotic Class - Essential for compliance testing

✅ Simplified Dairy Testing - Special milk protocol with trichloroacetic acid

✅ Time-Efficient - Complete analysis in<2.5 hours

✅ Cost-Effective - 96 tests/kit reduces per-sample cost

✅ Consistent Performance - ≤10% CV ensures reliable results

Technical Specifications:

Detection Limit: 2ppb (all matrices)

LOQ: 0.2ppb

Accuracy: 70-130% recovery

Precision: ≤10% CV

Format: 96-well microplate

Storage: 2-8°C (12 months)

Regulatory Compliance:

EU Regulation 37/2010

China GB/T 21317-2007

USDA Import Monitoring

Codex Alimentarius standards