FSTest Malachite Green ELISA Test Kit – Ultra-Sensitive Detection for Banned Aquaculture Chemicals

Introduction:

The FSTest Malachite Green ELISA Test Kit is a high-precision immunoassay designed for quantitative detection of malachite green (MG) and its metabolite leucomalachite green (LMG) in seafood samples.    With an exceptional 0.15ppb detection limit, this kit enforces compliance with global bans on these carcinogenic aquaculture chemicals.

Key Features & Benefits:

✔ Ultra-Sensitive Detection - 0.15ppb LOD (0.01ppb LOQ) meets strictest regulatory requirements

✔ Dual-Analyte Coverage - Detects both MG (100%) and LMG (20%)

✔ Regulatory Compliance - Supports enforcement of global MG bans in aquaculture

✔ Specialized Seafood Testing - Optimized for fish and shrimp matrices

✔ High-Throughput Format - 96-well design for efficient batch processing

✔ Validated Performance - ≤10% CV ensures reproducible results

Applications:

Seafood safety monitoring (fish, shrimp)

Aquaculture product export testing

Food import compliance verification

Regulatory enforcement programs

Testing Principle:

Competitive ELISA measuring absorbance at 450nm (inversely proportional to MG concentration) against a 6-point standard curve (0-0.08ppb).

How to Use:

1️⃣ Sample Preparation:

Homogenize seafood samples

Extract with acetonitrile/dichloromethane

Purify with neutral alumina

Oxidize with specialized reagents (fish: 30min;    shrimp: 30min)

2️⃣ Test Procedure:

Prepare fresh standard series (0-0.08ppb)

Add 100μL antibody solution, incubate 10min

Add 100μL sample/standard, incubate 30min

Add enzyme conjugate, incubate 15min

Add substrate, incubate 15min

Add stop solution

Read at 450nm

3️⃣ Result Interpretation:

Compare to standard curve

Apply 15× dilution factor

Why Choose FSTest Malachite Green ELISA Kit?

✅ Banned Substance Focus - Specifically designed for prohibited aquaculture chemicals

✅ Unmatched Sensitivity - Lowest detection limits in the industry

✅ Matrix-Specific Protocols - Different oxidation treatments for fish vs shrimp

✅ Regulatory Ready - Complies with EU/China/USFDA zero-tolerance policies

✅ Cost-Efficient - 96 tests/kit reduces per-sample cost

Technical Specifications:

Detection Limit: 0.15ppb

LOQ: 0.01ppb

Accuracy: 70-130% recovery

Cross-Reactivity: MG (100%), LMG (20%)

Format: 96-well microplate

Storage: 2-8°C (12 months)

Regulatory Compliance:

EU Regulation (EC) No 854/2004

China GB/T 20361-2006

USFDA Import Alert 16-131

Codex Alimentarius standards