FSTest Quinolones ELISA Test Kit – Comprehensive Antibiotic Residue Detection for Food Safety

Introduction:

The FSTest Quinolones ELISA Test Kit is a high-performance immunoassay designed for quantitative detection of 10 major quinolone antibiotics in animal tissues, seafood, eggs, honey, and milk.   With a 0.5ppb detection limit for most matrices (5ppb for milk), this kit provides reliable monitoring of these widely-used veterinary antibiotics to ensure food safety compliance.

Key Features & Benefits:

✔ Broad-Spectrum Detection - Covers 10 quinolones including enrofloxacin (100%), ciprofloxacin (110%), and norfloxacin (119%)

✔ Multi-Matrix Validation - Certified for 5 sample types with matrix-specific protocols

✔ Regulatory-Grade Sensitivity - 0.5ppb LOD meets global MRL requirements

✔ High-Throughput Format - 96-well design for efficient batch processing

✔ Optimized Workflow - Simplified extraction for different sample types

✔ Consistent Performance - ≤10% CV ensures reproducible results

Applications:

Livestock and poultry antibiotic monitoring

Aquaculture and seafood safety testing

Dairy and egg production quality control

Honey export compliance verification

Veterinary drug residue surveillance programs

Testing Principle:

Competitive ELISA measuring absorbance at 450nm (inversely proportional to quinolone concentration) against a 6-point standard curve (0-12ppb).

How to Use:

1️⃣ Sample Preparation:

Meat/Seafood/Eggs: Acetonitrile/dichloromethane extraction (3g sample)

Honey: Dual-phase extraction

Milk: Simple 10× dilution (100μL sample)

2️⃣ Test Procedure:

Add 50μL sample/standard

Add 50μL enzyme conjugate & antibody

Incubate 30min (20-28°C)

Wash 4-5 times

Add substrate, incubate 30min

Add stop solution

Read at 450nm

3️⃣ Result Interpretation:

Compare to standard curve (0.5-12ppb)

Apply matrix-specific dilution:

1× for tissue/seafood/eggs/honey

10× for milk

Why Choose FSTest Quinolones ELISA Kit?

✅ Most Comprehensive Coverage - Detects 10 key quinolones in one test

✅ Matrix-Specific Protocols - Optimized methods for each sample type

✅ Time-Efficient - Complete analysis in <2.5 hours

✅ Cost-Effective - 96 tests/kit reduces per-sample cost

✅ Regulatory Ready - Meets EU/China/USDA requirements

Technical Specifications:

Detection Limits:

Tissue/Seafood/Eggs/Honey: 0.5ppb

Milk: 5ppb

LOQ: 0.5ppb

Accuracy: 70-130% recovery

Precision: ≤10% CV

Format: 96-well microplate

Storage: 2-8°C (12 months)

Regulatory Compliance:

EU Maximum Residue Limits (MRLs)

China GB/T 20366-2006

USDA Food Safety Inspection

Codex Alimentarius standards