FSTest Chloramphenicol ELISA Test Kit – Ultra-Sensitive Detection for Banned Antibiotic Residues

Introduction:

The FSTest Chloramphenicol ELISA Test Kit is a high-precision immunoassay designed for quantitative detection of this prohibited antibiotic in animal tissues, seafood, honey, milk, and yogurt. With an exceptional 0.025ppb detection limit, this kit enforces compliance with global zero-tolerance regulations for chloramphenicol in food products.

Key Features & Benefits:

✔ Ultra-Sensitive Detection - 0.025ppb LOD in meat/seafood/honey, 0.1ppb in milk

✔ Regulatory Compliance - Meets EU/China/USDA zero-tolerance requirements

✔ Multi-Matrix Validation - Certified for 5 sample types including dairy

✔ High Specificity - 100% for CAP, <10% cross-reactivity (thiamphenicol)

✔ Efficient Workflow - 96-well format processes 20+ samples per run

✔ Ready-to-Use - Pre-coated plates and optimized reagents reduce setup time

Applications:

Meat/poultry safety monitoring

Seafood and honey export testing

Dairy product quality control

Veterinary drug surveillance programs

Food import/export compliance

Testing Principle:

Competitive ELISA measuring absorbance at 450nm (inversely proportional to CAP concentration) against a 6-point standard curve (0-1.2ppb).

How to Use:

1️⃣ Sample Preparation:

Meat/Seafood: Ethyl acetate extraction (3g sample)

Honey: Water/ethyl acetate dual-phase extraction

Dairy: Direct dilution (1-5mL)

2️⃣ Test Procedure:

Add 100μL sample/standard

Add 50μL enzyme conjugate & antibody

Incubate 30min (20-28°C)

Wash 4-5 times

Add substrate, incubate 30min

Add stop solution

Read at 450nm

3️⃣ Result Interpretation:

Compare to standard curve (0.05-1.2ppb)

Apply matrix-specific dilution factors:

0.5× for tissue/seafood/honey

2× for milk

5× for yogurt

Why Choose FSTest Chloramphenicol ELISA Kit?

✅ Gold-Standard Sensitivity - Lowest detection limits in industry

✅ Dairy-Specific Protocols - Unique milk/yogurt testing methods

✅ Regulatory Ready - Complies with global CAP bans

✅ Cost-Efficient - 96 tests/kit reduces per-sample cost by 40%

Technical Specifications:

Detection Limits:

Tissue/Seafood/Honey: 0.025ppb

Milk: 0.1ppb (0.05ppb with direct spotting)

Yogurt: 0.25ppb

LOQ: 0.05ppb

Accuracy: 70-130% recovery

Precision: ≤10% CV

Format: 96-well microplate

Storage: 2-8°C (12 months)

Regulatory Compliance:

EU Regulation 37/2010 (zero tolerance)

China GB/T 22338-2008

USDA Import Alert 16-04

Codex Alimentarius standards