FSTest Ractopamine ELISA Test Kit – High-Sensitivity Detection for Banned Growth Promoters

Introduction:

The FSTest Ractopamine ELISA Test Kit is a precision immunoassay designed for quantitative detection of ractopamine residues in animal tissues and liver samples.  With a 0.4ppb detection limit, this kit enforces compliance with global bans on this prohibited β-agonist growth promoter.

Key Features & Benefits:

✔ Ultra-Sensitive Detection - 0.4ppb LOD (0.1ppb LOQ) meets strictest regulatory requirements

✔ High Specificity - 100% for ractopamine,<1% cross-reactivity with other β-agonists

✔ Regulatory Compliance - Supports enforcement of EU/China bans on growth promoters

✔ Tissue-Specific Validation - Optimized for both muscle and liver matrices

✔ Efficient Workflow - 96-well format processes 20+ samples per run

✔ Ready-to-Use - Pre-coated plates and optimized reagents reduce setup time

Applications:

Meat safety monitoring (pork, beef)

Liver residue testing

Veterinary drug surveillance

Food import/export compliance

Regulatory enforcement programs

Testing Principle:

Competitive ELISA measuring absorbance at 450nm (inversely proportional to ractopamine concentration) against a 6-point standard curve (0-8.1ppb).

How to Use:

1️⃣ Sample Preparation:

Homogenize tissue/liver samples

Extract with HCl/NaCl/methanol solution

Centrifuge and pH adjust (7.8)

Dilute with sample diluent

2️⃣ Test Procedure:

Add 50μL sample/standard

Add 50μL enzyme conjugate & antibody

Incubate 30min (20-28°C)

Wash 4-5 times

Add substrate, incubate 15min

Add stop solution

Read at 450nm

3️⃣ Result Interpretation:

Compare to standard curve (0.1-8.1ppb)

Apply 4× dilution factor

Why Choose FSTest Ractopamine ELISA Kit?

✅ Banned Substance Focus - Specifically targets prohibited growth promoters

✅ Matrix-Specific Protocols - Different pH adjustment for tissue vs liver

✅ Regulatory Ready - Complies with global β-agonist bans

✅ Time-Efficient - Complete analysis in<2 hours

✅ Cost-Effective - 96 tests/kit reduces per-sample cost

Technical Specifications:

Detection Limit: 0.4ppb

LOQ: 0.1ppb

Accuracy: 70-130% recovery

Precision: ≤10% CV

Format: 96-well microplate

Storage: 2-8°C (12 months)

Regulatory Compliance:

EU Directive 96/22/EC

China Ministry of Agriculture Announcement No. 235

US FDA Compliance Policy Guide

Codex Alimentarius standards