FSTest Furazolidone Metabolite (AOZ) ELISA Test Kit – High Sensitivity, Accurate Quantification, and Fast Screening

The FSTest Furazolidone Metabolite (AOZ) ELISA Test Kit is a highly sensitive enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of furazolidone metabolites (AOZ) in animal tissues and seafood.  This test kit enables precise, low-detection limit results, making it ideal for food safety laboratories, regulatory agencies, and quality control departments.

Key Features & Benefits:

✔ Ultra-Low Detection Limit – 0.05ppb, ensuring trace-level AOZ residue detection.

✔ Accurate Quantification – Provides precise concentration measurements for compliance with international regulations.

✔ Simultaneous Batch Testing – 96-well plate format for high-throughput screening, increasing efficiency.

✔ Reliable & Reproducible – Coefficient of variation ≤ 10%, ensuring result consistency.

✔ Easy Sample Preparation – Compatible with animal tissue and seafood samples with a streamlined extraction protocol.

✔ Complies with Global Food Safety Standards – Ideal for EU, US FDA, and international regulatory requirements.

Applications:

Designed for rapid and accurate detection of AOZ residues in meat, poultry, fish, shrimp, and seafood products, ensuring food safety and compliance.

Testing Principle:

This competitive ELISA assay detects AOZ by measuring the color intensity at 450nm, which is inversely proportional to the AOZ concentration in the sample.  The absorbance is compared to a standard curve to determine precise AOZ levels.

How to Use:

1️⃣ Sample Preparation – Homogenize and extract the sample following the provided protocol.

2️⃣ Test Procedure:

Add 50μL of standard or sample to each test well.

Add 50μL of enzyme conjugate solution and 50μL of antibody solution.

Incubate at 20-28°C for 30 minutes in the dark.

Wash wells 4-5 times and remove excess liquid.

Add 100μL of substrate solution, incubate for 30 minutes, then add 100μL of stop solution.

Measure absorbance at 450nm using a microplate reader.

3️⃣ Result Interpretation – Compare absorbance values with the standard curve to determine AOZ concentration.

Why Choose FSTest AOZ ELISA Kit?

✅ Enhanced Detection Accuracy – Detects AOZ residues down to 0.05ppb, ensuring food safety.

✅ Optimized for Regulatory Compliance – Meets EU and US safety standards for food quality control.

✅ Efficient Sample Analysis – Capable of testing multiple samples in one run, reducing analysis time.

Storage & Stability:

Store at 2-8°C in a sealed container.  Shelf life: 12 months.  Do not freeze.

Ensure food safety compliance with the FSTest Furazolidone Metabolite (AOZ) ELISA Test Kit – the leading solution for highly sensitive, precise, and cost-effective AOZ residue testing.