FSTest Sulfonamides ELISA Test Kit – High Sensitivity, Accurate Quantification, and Regulatory Compliance

Introduction:

The FSTest Sulfonamides ELISA Test Kit is a highly sensitive enzyme-linked immunosorbent assay designed for the quantitative detection of sulfonamide residues in animal tissues and seafood. With a detection limit of 2ppb, this kit provides reliable screening to ensure compliance with international food safety regulations.

Key Features & Benefits:

✔ Broad-Spectrum Detection – Covers 11 major sulfonamides including Sulfadiazine (100%), Sulfamethoxazole (96%), and Sulfaguanidine (98%)

✔ Regulatory-Grade Sensitivity – 2ppb detection limit meets strict food safety requirements

✔ High-Throughput Screening – 96-well format enables efficient batch processing

✔ Reliable Performance – ≤10% coefficient of variation ensures reproducible results

✔ Simplified Workflow – Optimized extraction protocol for animal tissues and seafood

✔ Global Compliance – Meets international standards for sulfonamide residue testing

Applications:

Meat and poultry testing (chicken, pork)

Seafood safety monitoring (fish, shrimp)

Food import/export compliance

Veterinary drug residue screening

Testing Principle:

This competitive ELISA measures absorbance at 450nm, which is inversely proportional to sulfonamide concentration. Results are quantified against a standard curve (0-80ppb range).

How to Use:

1️⃣ Sample Preparation:

Homogenize tissue/seafood samples

Extract with ethyl acetate, evaporate, and reconstitute

2️⃣ Test Procedure:

Add 100μL sample/standard to wells

Add 50μL enzyme conjugate

Incubate 30min at 20-28°C

Wash 4-5 times

Add substrate, incubate 30min

Add stop solution

Measure absorbance at 450nm using a microplate reader.

3️⃣ Result Interpretation – Compare absorbance values with the standard curve to determine Result.

Why Choose FSTest Sulfonamides ELISA Kit?

✅ Comprehensive Detection – Covers key sulfonamides with varying cross-reactivity

✅ Validated Accuracy – 70-130% recovery rate across sample types

✅ Cost-Effective – 96-well format reduces per-test cost

✅ Regulatory Ready – Supports compliance with global food safety standards

Storage & Stability:

Store at 2-8°C. Shelf life: 12 months. Do not freeze.

Technical Specifications:

Detection Limit: 2ppb

Sample Types: Animal tissues, seafood

Format: 96-well microplate

Standard Range: 0, 2, 5, 10, 30, 80ppb

Ensure food safety compliance with the FSTest Sulfonamides ELISA Test Kit – your trusted solution for accurate, efficient sulfonamide residue detection.