Product Description
The FSTest Ractopamine Rapid Test Kit is based on sandwich lateral flow immunochromatography assay. The test cassette has a window for the observation of assay running and result reading. The window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample well on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated recombinant antigens. If there is ractopamine present in the sample, the T line will become visible. The C line should always appear after a sample is applied, which indicates a valid result .By this way, the device can indicate the presence of ractopamine in the sample.
Application
Intended to test ractopamine in pork, chicken, duck, beef, mutton and other muscle tissues.
Limit of detection
0.5 μg/kg
Kit composition
Test cassettes ×10
Ractopamine extracting solution ×1
15 mL Centrifuge tubes x 10
User manual.
Specifications
10 tests
Sample preparation
1. Make the homogenized meat sample with only tissues and
avoid fats.
2. Weigh 4 g of the sample into a 15 mL centrifuge tube, mix with
1 mL of extracting solution and vortex or shake over for 2 min.
3. Bathe the centrifuge tube in 80℃ water for 10 min. Note: Ensure the water level of the bathe surpasses the sample
liquid inside of the centrifuge tube. Be aware of water leaks into the
tube.
4. Centrifuge at 4000rpm for 5 min. Cool the supernatant to room temperature. The sample solution is ready for testing.
Procedure & Steps
1. Before use, ensure the cassette is between 20 to 40℃, and put
it on a flat surface.
2. Pipette 100 μL of sample solution into a microwell, mix 3-5
times with pipette, then set for 3 min.
3. Transfer all the mixed solution and mount it into the sample hole of the test cassette.
4. Interpret the results after 5-8 min. Results are invalid after 8 min.