8–10 mins Rapid Readout Time | 100 µg/kg Combined Total LOD | 10 Tests Complete Kit Pack | 12 Months Shelf Life (4–30°C) |
The FSTest Enrofloxacin and Ciprofloxacin Rapid Test Kit utilizes immunochromatography for the qualitative detection of total residues of enrofloxacin and ciprofloxacin in seafood, livestock and poultry tissues, and poultry egg samples.
As broad-spectrum quinolone antibacterials with strong tissue penetration, enrofloxacin and ciprofloxacin are widely used in veterinary medicine; however, potential drug resistance and toxicity necessitate strict regulatory monitoring. This kit provides a simple, rapid, and visual screening solution suitable for on-site quality verification by food enterprises, testing laboratories, and regulatory agencies.
Dual Target Total Screening: Detects combined total residues of enrofloxacin and ciprofloxacin in a single rapid assay loop.
Broad Matrix Application: Optimized across diverse sample types including livestock tissues, poultry tissues, seafood products, and poultry eggs.
Rapid & Visual Interpretation: Clear visual results directly observed by the naked eye within 8–10 minutes.
User-Friendly Operation: Streamlined workflow featuring convenient test cassettes and microwells for field or laboratory rapid testing.
Convenient Room Temp Storage: Offers a full 12-month shelf life when sealed and stored at 4–30°C in a cool, dry place.
| Product Name | Enrofloxacin and Ciprofloxacin Rapid Test Kit |
|---|---|
| Detection Principle | Immunochromatography Assay |
| Pack Size | 10 tests / kit |
| Assay Time & Sample Volume | 8–10 minutes (results invalid after 15 minutes); 100 µL test solution |
| Storage & Shelf Life | 12 months sealed at 4–30°C in a cool, dry place (Do not freeze) |
| Target Analytes (In Total) | Detection Principle | LOD Threshold (µg/kg) |
|---|---|---|
| Enrofloxacin & Ciprofloxacin (Total) | Immunochromatography | 100 µg/kg (In total) |
Optimized for multi-matrix quinolone screening across various food categories:
STEP 1 Extraction Homogenize >100g sample. Weigh 1.0g into 15mL tube, add 5mL Extraction Solution, cap tightly, shake vigorously for 1 min until dispersed/flocculent. | STEP 2 Dilution Centrifuge at 4000 rpm for 5 min. Transfer 100µL supernatant to 2mL tube, add 900µL Dilution Solution, and mix thoroughly for 30 seconds. | STEP 3 Load Cassette Ensure cassette is at 20–40°C. Pipette 100µL solution into microwell, mix 3–5 times, let stand 3 min. Transfer all solution into cassette sample well. | STEP 4 Read at 8–10 Mins Read visual lines strictly at 8–10 minutes. Results become invalid after 15 minutes due to post-reaction shifts. |
Negative (-): Both Control (C) line and Test (T) line appear distinctly. Combined enrofloxacin and ciprofloxacin concentration is below 100 µg/kg.
Positive (+): Control (C) line appears, while Test (T) line does not appear → Total enrofloxacin and ciprofloxacin concentration ≥ 100 µg/kg.
Invalid: Control (C) line fails to appear, regardless of T line presence. Re-testing with a new cassette is required.
Adhere to strict operational parameters to ensure maximum screening accuracy:
Qualitative Screening Purpose: Engineered strictly for qualitative preliminary screening; not intended for precise quantitative concentration evaluation.
Temperature Equilibrate: Ensure the test cassette and reagents are equilibrated to 20–40°C before performing the test.
Strict Time Window: Read results strictly between 8–10 minutes after loading the cassette; results observed after 15 minutes are invalid.
Kit Components Included: 10 Test cassettes, 10 Microwells, Extracting solution (60mL) x1, Diluent solution (10mL) x1, 15mL Centrifuge tubes (x10), 2mL Centrifuge tubes (x10), User Manual.
Required Equipment (Not Provided): Homogenizer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, vortexer, and timer.